Isotope Tracers in Metabolic Research
| Announcing a New Course Presented by Mouse Metabolic Phenotyping Centers and the
UAMS Donald W. Reynolds Institute on Aging |
| Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis |
October 8-12, 2007
University of Arkansas for Medical Sciences
Donald W. Reynolds Institute on Aging
Little Rock, Arkansas
| A week-long course in the theory and practice of isotopic tracers (stable and radioactive)
for the study of metabolism in man and animals using mass spectrometry and NMR. The course
will emphasize isotopomer analysis for metabolic flux rates and metabolic regulation. |
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Director & Faculty Information
| Course Director: | Robert Wolfe, PhD |
| Co-Director: | Henri Brunengraber, MD, PhD |
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| Faculty: |
| Gianni Biolo, MD | Trieste, Italy |
| Henri Brunengraber, MD, PhD | Cleveland, OH |
| Shawn Burgess, PhD | Dallas, TX |
| Gary Cline, PhD | New Haven, CT |
| Nicolaas E. Deutz, MD, PhD | Little Rock, AR |
| Arny Ferrando, PhD | Little Rock, AR |
| Joanne Kelleher, PhD | Boston, MA |
| Maren Laughlin, PhD | Bethesda, MD |
| Craig Malloy, MD | Dallas, TX |
| Dwight Matthews, PhD | Burlington, VT |
| Owen McGuinness, PhD | Nashville, TN |
| Stephen Previs, PhD | Cleveland, OH |
| Olav Rooyackers, PhD | Stockholm, Sweden |
| Robert Wolfe, PhD | Little Rock, AR |
Sponsored by:
Mouse Metabolic Phenotyping Centers
University of Arkansas for Medical Sciences Donald W. Reynolds Institute on Aging
The National Institute of Diabetes & Digestive & Kidney Diseases
The National Heart, Lung & Blood Institute
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Course Information
Course Agenda:
Click here to download course syllabus.
Course Materials:
Materials will be provided in paper and/or CD at the course. Participants may wish to bring a laptop
for homework problems. In addition, the following materials are highly recommended:
- “Isotope Tracers in Metabolic Research: Principle and Practice of Kinetic Analysis” by Robert R. Wolfe and David L. Chinkes, 2nd Edition, 2005. Wiley.
- Literature on NMR Isotopomer Analysis (citations to be provided)
- Two software programs, tcaSIM and tcaCALC, are available for download. To download the software, go to Download Registration and complete the
registration form. The tcaSim program is a tool for use with labeling studies in intermediary metabolism - primarily those applying 13C NMR spectroscopy,
but also for 14C radiolabel studies and 13C mass isotopomer analysis. The simulation has been developed to assist in the design of experiments and to help the
user understand the effect of various metabolic states and substrate enrichment levels on labeling patterns. The tcaCALC program performs an isotopomer analysis
to estimate relative pathway fluxes in biological systems at metabolic and isotopic steady state. It is used in 13C labeling studies where biological systems (isolated
cells, perfused organs or intact animals) are provided enriched substrate(s), and the labeling of intermediates detected using 13C NMR spectroscopy, mass- and tandem
mass spectrometry. The 13C spectra are collected either directly from intact tissue, such as in the case of perfusions conducted inside a magnet, or after extraction
of a tissue sample (isolated cell studies or tissue samples taken after in vivo infusion of 13C-enriched substrates).
Logistics:
Classes will be held at the Donald W. Reynolds Institute on Aging, Ground Floor, 629 Jack Stephens Blvd,
on the UAMS campus. Please plan to arrive Sunday night, Oct. 7, 2007, and to leave after noon on Friday,
Oct. 12. The airport is 15-20 minutes from the campus and hotels.
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Registration & Accomodations
Registration Fee:
Breakfast, lunch and course materials are included in the registration fee. Limited to 150 participants.
| Students / Post docs | $300 |
| Academic / Government scientists | $700 |
| Industry scientists | $1200 |
Registration Form:
Click here to download Registration Form.
Please print and complete the form and mail it with a check made out to “Tracer Methodology Course”, to:
Deborah Viane, 4301 W. Markham St., Slot 806, Little Rock, AR 72205-7199. Registration and fees must be
received by September 15, 2007.
Parallel Sessions:
Please indicate on the registration form which tracks you are interested in attending on Wednesday and
Thursday evening. This is for planning purposes only.
Accommodations:
A limited number of rooms will be reserved at special rates for “The Tracer Course” at Embassy Suites.
Reservations should be made by September 1, 2007.
Embassy Suites
11301 Financial Centre Parkway
(501) 312-9000
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Contact Information
Deborah Viane
University of Arkansas For Medical Research
4301 W. Markham St., Slot 806
Little Rock, AR 72205-7199
Phone: 501-526-5709
Fax: 501-526-5710
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