Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived
Cardiomyocytes: Matrigel Mattress Technique.
Authors Cadar AG, Feaster TK, Durbin MD, Hong CC
Submitted By Submitted Externally on 9/13/2017
Status Published
Journal Current protocols in stem cell biology
Year 2017
Date Published 8/1/2017
Volume : Pages 42 : 4A.14.1 - 4A.14.7
PubMed Reference 28806851
Abstract This unit describes the published Matrigel mattress method. Briefly, we describe
the preparation of the mattress, replating of the human induced pluripotent stem
cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM
mattress maintenance. Adherence to this protocol will yield individual, robustly
shortening hiPSC-CMs, which can be used for downstream applications. © 2017 by
John Wiley & Sons, Inc.