Migration pathways of sacral neural crest during development of lower urogenital
tract innervation.
Authors Wiese CB, Deal KK, Ireland SJ, Cantrell VA, Southard-Smith EM
Submitted By Submitted Externally on 9/13/2017
Status Published
Journal Developmental biology
Year 2017
Date Published 9/1/2017
Volume : Pages 429 : 356 - 369
PubMed Reference 28449850
Abstract The migration and fate of cranial and vagal neural crest-derived progenitor
cells (NCPCs) have been extensively studied; however, much less is known about
sacral NCPCs particularly in regard to their distribution in the urogenital
system. To construct a spatiotemporal map of NCPC migration pathways into the
developing lower urinary tract, we utilized the Sox10-H2BVenus transgene to
visualize NCPCs expressing Sox10. Our aim was to define the relationship of
Sox10-expressing NCPCs relative to bladder innervation, smooth muscle
differentiation, and vascularization through fetal development into adulthood.
Sacral NCPC migration is a highly regimented, specifically timed process, with
several potential regulatory mileposts. Neuronal differentiation occurs
concomitantly with sacral NCPC migration, and neuronal cell bodies are present
even before the pelvic ganglia coalesce. Sacral NCPCs reside within the pelvic
ganglia anlagen through 13.5 days post coitum (dpc), after which they begin
streaming into the bladder body in progressive waves. Smooth muscle
differentiation and vascularization of the bladder initiate prior to innervation
and appear to be independent processes. In adult bladder, the majority of Sox10+
cells express the glial marker S100ß, consistent with Sox10 being a glial marker
in other tissues. However, rare Sox10+ NCPCs are seen in close proximity to
blood vessels and not all are S100ß+, suggesting either glial heterogeneity or a
potential nonglial role for Sox10+ cells along vasculature. Taken together, the
developmental atlas of Sox10+ NCPC migration and distribution profile of these
cells in adult bladder provided here will serve as a roadmap for future
investigation in mouse models of lower urinary tract dysfunction.