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Pilot & Feasibility Program Application Abstract
Live-in gradient layer calorimetry for high throughput metabolic studies of genetically altered mice
Karl Kaiyala   (Seattle, WA)
Genetically altered mice play major roles in basic obesity research and in efforts to discover anti-obesity drugs. Some obesity-causing genotypes, including a novel one described herein, may involve a subtle defect in metabolic energy expenditure. Accurately characterizing metabolic phenotypes requires sensitive, long-duration measurements of metabolic rate in unstressed mice. However, metabolic rate is almost always quantified via indirect calorimetry, an expensive, complex and fragile methodology with considerable (but often hidden) error-favoring pitfalls. Gradient layer calorimetry (GLC) affords an inexpensive, robust and sensitive alternative for measuring metabolic rate in mice. The latter would permit high-throughput studies in unstressed mice, which could be a valuable means for efficiently assessing the metabolic consequences of genetic alterations. We propose such a system, and seek to use it to study AC3-/- obese mice.

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