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Pilot & Feasibility Program Application Abstract
Assessment of Diastolic Heart Function in Diabetic Mice
Elina Minami   (Seattle, WA)
This proposal's main objective is to develop a non-invasive method of studying diastolic heart function in diabetic mice using tissue doppler imaging, which is a non-invasive, high throughput method so that experimental animals can be followed over time. Currently, the gold standard of studying diastolic heart function is by invasive left heart catheterization, which is extremently labor-intensive and is a terminal experiment. This method only allows the investigator to look at the diastolic and systolic function of the heart at the time of study. Tissue doppler imaging has been utilized in the clinical setting and has been validated in mice models as a reliable method to study regional myocardial function. Diastolic dysfunction is predominant in diabetes mellitus and is one of the contributors to poor outcomes following myocardial infarction. In addition, diastolic cardiomyopathy is a common occurence in patients with diabetes mellitus independent of hypertension and coronary heart disease. The causes of this increased stiffness, impaired relaxation, and increased fibrosis are still not fully elucidated. By using a myocardial infarction model in diabetic mice, the proposal will focus on the following Aims to better understand and to develop another method of studying this process: 1) Compare infarct repair between Type I and Type 2 diabetic mice models following myocardial infarction 2) Assess the difference in diastolic function between the two diabetic mice models by left ventricular catheterization and pressure-volume loops 3) Develop a high throughput, non-invasive assessment of diastolic function and myocardial stiffness by myocardial strain rate using tissue Doppler imaging.

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