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Experiment

Analytical Study - Plasma Leptin Levels in Adiponectin-Raptor KO Mice
Summary Data Summary
Investigator Guertin, David
Description Normal adipose tissue growth and function is critical to maintaining metabolic
homeostasis and its excess (e.g. obesity) or absence (e.g. lipodystrophy) is
associated with severe metabolic disease. The goal of this study was to
understand the mechanisms maintaining healthy adipose tissue growth and
function.

Adipose tissue senses and responds to systemic changes in growth factor and
nutrient availability; in cells mTORC1 regulates metabolism in response to
growth factors and nutrients. Thus, mTORC1 is poised to be a critical
intracellular regulator of adipocyte metabolism. Here, we investigate the role
of mTORC1 in mature adipocytes by generating and characterizing mice in which
the Adiponectin-Cre driver is used to delete floxed alleles of Raptor, which
encodes an essential regulatory subunit of mTORC1

Adiponectin-Cre driven adipose specific KO of Raptor. KO mice do not expand
adipose tissue depots, and are lipodystrophic.
Status Completed
Public Release 4/23/2018
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame StrainExperiment Description
Data Analysis
TypeCount
Animals18
Experimental Conditions2
Catalog Items1
Curation Info (# flags)2
Phenotype Assays1
Phenotype Measurements18
Histology Images0
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6J +/+
0
9
0
adipose-specific raptor knockout
0
9
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Mouse Diet

 LabDiet 5P75/5P76
Prolab 5P75 and 5P76 Isopro 3000 are the irradiated forms of RMH 3000 (5P00). The product has been irradiated to virtually bacteria-free dietary control. Protien 22.5%, Fat 5.4% Cholesterol 195 ppm
 Teklad - 93075
Harlan-Teklad TD.93075 Adjusted Calorie diet (Calories: 21% protein, 55% Fat, 24% Carbohydrate) A diet used to induce obesity. Approximate fatty acid profile (% total fat): 28% saturated, 30% trans, 28% monounsaturated (cis), 14% polyunsaturated...
Experimental Factor (Units)
Experimental Group
Mouse Diet


Phenotype Assays Add / Edit



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