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Publication
Delivery of recombinant SARS-CoV-2 envelope protein into human cells.
Authors Hutchison JM, Capone R, Luu DD, Hadziselimovic A, Van Horn WD, Sanders CR
Submitted By Submitted Externally on 12/3/2021
Status Published
Journal bioRxiv : the preprint server for biology
Year 2021
Date Published 2/1/2021
Volume : Pages Not Specified : Not Specified
PubMed Reference 33619482
Abstract SARS-CoV-2 envelope protein (S2-E) is a conserved membrane protein that is
essential to coronavirus assembly and budding. Here, we describe the recombinant
expression and purification of S2-E into amphipol-class amphipathic polymer
solutions. The physical properties of amphipols underpin their ability to
solubilize and stabilize membrane proteins without disrupting membranes.
Amphipol delivery of S2-E to pre-formed planar bilayers results in spontaneous
membrane integration and formation of viroporin ion channels. Amphipol delivery
of the S2-E protein to human cells results in membrane integration followed by
retrograde trafficking to a location adjacent to the endoplasmic
reticulum-to-Golgi intermediate compartment (ERGIC) and the Golgi, which are the
sites of coronavirus replication. Delivery of S2-E to cells enables both
chemical biological approaches for future studies of SARS-CoV-2 pathogenesis and
development of "Trojan Horse" anti-viral therapies. This work also establishes a
paradigm for amphipol-mediated delivery of membrane proteins to cells.




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