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Publication
Intestinal Lymph Collection via Cannulation of the Mesenteric Lymphatic Duct in
Mice.
Authors Banan B, Wei Y, Simo O, Tso P, Abumrad NN, Flynn CR, Sundaresan S, Albaugh VL
Submitted By Submitted Externally on 12/3/2021
Status Published
Journal The Journal of surgical research
Year 2021
Date Published 4/1/2021
Volume : Pages 260 : 399 - 408
PubMed Reference 33261855
Abstract We have optimized a technique for cannulation of mesenteric lymph duct (MLD) in
mice. Mice have low rates of intestinal lymph production; the MLDs are smaller
and associated with fragile vasculature. Previous protocols for lymph collection
based on the open lymph fistula model were associated with low success rates in
mice. Bariatric surgery procedures worsen success rates due to postoperative
adhesions and GI rearrangement. We have used this procedure to collect
mesenteric lymph from mice undergoing bile diversion from gall bladder to ileum
(GB-IL)., We hypothesize that peptide YY (PYY) levels in mesenteric lymph will
increase following nutrient delivery in mice undergoing bile diversion from gall
bladder to ileum (GB-IL)., We observe that cannulation of the MLD using a
needled-catheter maintains lymph vessel integrity, prevents excessive lymph
leakage, and is less traumatic, leading to high success rates (>95%). PYY levels
in mesenteric lymph after GB-IL were significantly higher post nutrient
infusion. The procedure takes approximately 20 min; small rodent surgical
experience and practice are required for success., Intestinal lymph can be
collected from mice, including those undergoing bariatric surgical procedures
with high success rates by cannulation of the mesenteric lymph duct.




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