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Publication
Accelerated protocol for the differentiation of podocytes from human pluripotent
stem cells.
Authors Bejoy J, Qian ES, Woodard LE
Submitted By Submitted Externally on 3/9/2022
Status Published
Journal STAR protocols
Year 2021
Date Published 12/1/2021
Volume : Pages 2 : 100898
PubMed Reference 34746862
Abstract Several kidney diseases including congenital nephrotic syndrome, Alport
syndrome, and diabetic nephropathy are linked to podocyte dysfunction. Human
podocytopathies may be modeled in either primary or immortalized podocyte cell
lines. Human induced pluripotent stem cell (hiPSC)-derived podocytes are a
source of human podocytes, but the existing protocols have variable efficiency
and expensive media components. We developed an accelerated, feeder-free
protocol for deriving functional, mature podocytes from hiPSCs in only 12 days,
saving time and money compared with other approaches.




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