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Measurement of Hepatic Lipids.
Authors Forman S, Tso P, Liu M
Submitted By Submitted Externally on 6/21/2022
Status Published
Journal Methods in molecular biology (Clifton, N.J.)
Year 2022
Date Published
Volume : Pages 2455 : 41 - 48
PubMed Reference 35212984
Abstract Liver plays a central role in lipid metabolism, uptake of lipoproteins and
lipids from the circulation (e.g., chylomicron remnant), and secretions of very
low-density lipoproteins (VLDL). Therefore, measurements of lipid levels in the
liver have been broadly used to check hepatic function, especially in subjects
who have chronic liver diseases, such as nonalcoholic steatohepatitis (NASH), in
which there is accumulation of fat, inflammation, and damage to liver cells. In
this chapter, we describe the processes of extracting hepatic lipids by the
method of Folch et al., and measuring the levels of cholesterol, triglycerides,
phospholipids, and non-esterified fatty acids using enzymatic assays.


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