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Publication
Evaluation of Dying Vocal Fold Epithelial Cells by Ultrastructural Features and
TUNEL Method.
Authors Novaleski CK, Mizuta M, Rousseau B
Submitted By Submitted Externally on 12/21/2016
Status Published
Journal Cells, tissues, organs
Year 2016
Date Published
Volume : Pages 202 : 355 - 368
PubMed Reference 27537846
Abstract Cell death is a regulated mechanism of eliminating cells to maintain tissue
homeostasis. This study described 2 methodological procedures for evaluating
cell death in the epithelium of immobilized, approximated and vibrated vocal
folds from 12 New Zealand white breeder rabbits. The gold standard technique of
transmission electron microscopy evaluated high-quality ultrastructural criteria
of cell death and a common immunohistochemical marker, the terminal
deoxynucleotidyl transferase dUTP nick end labeling method, to confirm cell
death signaling. Results revealed that ultrastructural characteristics of
apoptotic cell death, specifically condensed chromatin and apoptotic bodies,
were observed after vocal fold vibration and approximation. Although episodes of
necrosis were rare, few enlarged cell nuclei were present after vibration and
approximation. The vocal fold expresses an immunohistochemical marker for
apoptosis along the apical surface of the epithelium. This study provides a
solid foundation for future investigations regarding the role of cell death in
vocal fold health and disease.




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