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Publication
p73 Is Required for Ovarian Follicle Development and Regulates a Gene Network
Involved in Cell-to-Cell Adhesion.
Authors Santos Guasch GL, Beeler JS, Marshall CB, Shaver TM, Sheng Q, Johnson KN, Boyd
KL, Venters BJ, Cook RS, Pietenpol JA
Submitted By Submitted Externally on 11/5/2018
Status Published
Journal iScience
Year 2018
Date Published 10/1/2018
Volume : Pages 8 : 236 - 249
PubMed Reference 30340069
Abstract We report that p73 is expressed in ovarian granulosa cells and that loss of p73
leads to attenuated follicle development, ovulation, and corpus luteum
formation, resulting in decreased levels of circulating progesterone and defects
in mammary gland branching. Ectopic progesterone in p73-deficient mice
completely rescued the mammary branching and partially rescued the ovarian
follicle development defects. Performing RNA sequencing (RNA-seq) on transcripts
from murine wild-type and p73-deficient antral follicles, we discovered
differentially expressed genes that regulate biological adhesion programs.
Through modulation of p73 expression in murine granulosa cells and transformed
cell lines, followed by RNA-seq and chromatin immunoprecipitation sequencing, we
discovered p73-dependent regulation of a gene set necessary for cell adhesion
and migration and components of the focimatrix (focal intra-epithelial matrix),
a basal lamina between granulosa cells that promotes follicle maturation. In
summary, p73 is essential for ovarian folliculogenesis and functions as a key
regulator of a gene network involved in cell-to-cell adhesion and migration.




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