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Publication
Attenuation of diet-induced hypothalamic inflammation following bariatric
surgery in female mice.
Authors Herrick MK, Favela KM, Simerly RB, Abumrad NN, Bingham NC
Submitted By Submitted Externally on 11/16/2018
Status Published
Journal Molecular medicine (Cambridge, Mass.)
Year 2018
Date Published 10/1/2018
Volume : Pages 24 : 56
PubMed Reference 30355312
Abstract Exposure of rodents to chronic high-fat diet (HFD) results in upregulation of
inflammatory markers and proliferation of microglia within the mediobasal
hypothalamus. Such hypothalamic inflammation is associated with metabolic
dysfunction, central leptin resistance, and maintenance of obesity. Bariatric
surgeries result in long-term stable weight loss and improved metabolic
function. However, the effects of such surgical procedures on HFD-induced
hypothalamic inflammation are unknown. We sought to characterize the effects of
two bariatric surgical procedures, Roux-en-Y gastric bypass (RYGB) and biliary
diversion (BD-IL), in female mice with particular emphasis on HFD-induced
hypothalamic inflammation and microgliosis., RYGB and BD-IL were performed on
diet-induced obese (DIO) mice. Quantitative RT-PCR and fluorescent microscopy
were used to evaluate hypothalamic inflammatory gene expression and
microgliosis. Results were compared to lean (CD), DIO sham-surgerized mice
(DIO-SHAM), and dietary weight loss (DIO-Rev) controls., In female mice, RYGB
and BD-IL result in normalization of hypothalamic inflammatory gene expression
and microgliosis within 8 weeks of surgery, despite ongoing exposure to HFD.
Paralleling these results, the hypothalamic expression levels of the orexigenic
neuropeptide Agrp and the anorexic response of surgical mice to exogenous leptin
were comparable to lean controls (CD). In contrast, results from DIO-Rev mice
were comparable to DIO-SHAM mice, despite transition back to standard rodent
show and normalization of weight., Bariatric surgery attenuates HFD-induced
hypothalamic inflammation and microgliosis and restores leptin sensitivity,
despite ongoing exposure to HFD.




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