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Use of Isotope Tracers to Assess Lipid Absorption in Conscious Lymph Fistula
Authors Ko CW, Qu J, Liu M, Black DD, Tso P
Submitted By Submitted Externally on 2/28/2019
Status Published
Journal Current protocols in mouse biology
Year 2019
Date Published 2/1/2019
Volume : Pages Not Specified : e60
PubMed Reference 30801996
Abstract This protocol provides a comprehensive reference for the evolution of the lymph
fistula model, the mechanism of lipid absorption, the detailed procedure for
studying lipid absorption using the lymph fistula model, the interpretation of
the results, and consideration of the experimental design. The lymph fistula
model is an approach to assess the concentration and rate of a range of
molecules transported by the lymph by cannulating lymph duct in animals. In this
protocol, mice first undergo surgery with the implantation of cannulae in the
duodenum and mesenteric lymph duct and are allowed to recover overnight in
Bollman restraining cages housed in a temperature-regulated environment. To
study in vivo lipid absorption, a lipid emulsion is prepared with labeled
tracers, including [3 H]-triolein and [14 C]-cholesterol. On the day of the
experiment, mice are continuously infused with lipid emulsion via the duodenum
for 6 hr, and lymph is usually collected hourly. At the end of the study,
gastrointestinal segments and their luminal contents are collected separately
for determination of the digestion, uptake, and transport of exogenous lipids. ©
2019 by John Wiley & Sons, Inc.


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