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Dietary Methionine Restriction Reduces Inflammation Independent of FGF21 Action.
Sharma S, Dixon T, Jung S, Graff EC, Forney LA, Gettys TW, Wanders D
Submitted Externally on 6/24/2019
Obesity (Silver Spring, Md.)
Volume : Pages
Methionine restriction (MR) decreases inflammation and improves markers of
metabolic disease in rodents. MR also increases hepatic and circulating
concentrations of fibroblast growth factor 21 (FGF21). Emerging evidence has
suggested that FGF21 exerts anti-inflammatory effects. The purpose of this study
was to determine the role of FGF21 in mediating the MR-induced reduction in
inflammation., Wild-type and Fgf21-/- mice were fed a high-fat (HF) control or
HF-MR diet for 8 weeks. In a separate experiment, mice were fed a HF diet (HFD)
for 10 weeks. Vehicle or recombinant FGF21 (13.6 µg/d) was administered via
osmotic minipump for an additional 2 weeks. Inflammation and metabolic
parameters were measured., Fgf21-/- mice were more susceptible to HFD-induced
inflammation, and MR reduced inflammation in white adipose tissue (WAT) and
liver of Fgf21-/- mice. MR downregulated activity of signal transducer and
activator of transcription 3 in WAT of both genotypes. FGF21 administration
reduced hepatic lipids and blood glucose concentrations. However, there was
little effect of FGF21 on inflammatory gene expression in liver or adipose
tissue or circulating cytokines., MR reduces inflammation independent of FGF21
action. Endogenous FGF21 is important to protect against the development of
HFD-induced inflammation in liver and WAT, yet administration of low-dose FGF21
has little effect on markers of inflammation.
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Financial support for this work was provided by the NIDDK Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997,
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