MMPC :: Catalog Item

Fatty acid and cholesterol synthesis using 2H-labeled water

Center	Case Western Reserve University
Center Core	Analytical Core
Test No.	CA2016
Test Price	$75.00 / sample
Description	Rates of fatty acid and cholesterol synthesis can be determined in tissues via the incorporation of 2H or 13C-labeled precursors. For example, following a bolus injection of 2H-labeled water one can collect samples (e.g. blood, liver and/or adipose tissue). The respective lipids are isolated and their 2H-labeling is determined. This test can be performed in 2 modes, short term vs long term. In a short term study, the tracer is administered and samples are collected within hours to determine the synthesis of lipids in plasma and/or liver. In a long term study, the tracer is continuously administered over several days. Samples of adipose tissue are collected. The difference in time scale is necessary since the pool of lipids in adipose tissue is relatively large and requires more time for label to appear. Note: This test does not require catheterized mice, nor does it require that mice be shipped to the MMPC. The isotopes are non-radioactive and no special safety precautions are required, the tracers will be shipped from the MMPC to the investigator. Investigators will be instructed on how to administer the isotopes, collect samples and then ship them back to the MMPC.
Data	No data has been collected for this catalog item
Keywords

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Lipid Metabolism

No phenotype assays found.