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Catalog Item
Fatty acid and/or cholesterol synthesis using 2H-labeled water includes test:CA2016A, see below
Common Name(s): De Novo Lipogenesis

CenterCase Western Reserve University
Center CoreAnalytical Core
Test No.CA2016
Test Price$150.00/test
DescriptionRates of fatty acid and cholesterol synthesis can be determined in tissues via
the incorporation of 2H or 13C-labeled precursors. For example, following a
bolus injection of 2H-labeled water one can collect samples (e.g. blood, liver
and/or adipose tissue). The respective lipids are isolated and their 2H-labeling
is determined. This test can be performed in 2 modes, short term vs long term.
In a short term study, the tracer is administered and samples are collected
within hours to determine the synthesis of lipids in plasma and/or liver. In a
long term study, the tracer is continuously administered over several days.
Samples of adipose tissue are collected. The difference in time scale is
necessary since the pool of lipids in adipose tissue is relatively large and
requires more time for label to appear.

Note: This test does not require catheterized mice, nor does it require that
mice be shipped to the MMPC. The isotopes are non-radioactive and no special
safety precautions are required, the tracers will be shipped from the MMPC to
the investigator. Investigators will be instructed on how to administer the
isotopes, collect samples and then ship them back to the MMPC.
DataData will be collected for this catalog item
Keywordscholesterol, fatty acids, glucose metabolism, insulin, insulin action, insulin
resistance, lipids, obesity
Tissuesadipocyte

No catalog groups / subjects found.



Phenotype Assays
NameAbbreviationOptions
palmitate, TG-bound, newly sythesized
TG-bound palmitate N
View
palmitate, TG-bound new
TG-bound plamitate n
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palmitate, TG-bound total
TG-bound palmitate t
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glycerol, total labeled
glycerol total label
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glycerol, TG-bound total
TG-bound glycerol to
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glycerol, TG-bound new
TG-bound glycerol ne
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triglyceride, newly synthesized
new triglyceride
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cholesterol, newly synthesized
new cholesterol
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cholesterol, TG-bound total
TG-bound cholesterol
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cholesterol, TG-bound new
TG-bound cholesterol
View
triglyceride, TG-bound new
TG-bound triglycerid
View
triglyceride, TG-bound total
TG-bound triglycerid
View




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