Catalog Item
Activity of acetyl-CoA carboxylase or malonyl-CoA decarboxylase in tissues

CenterCase Western Reserve University
Center CoreAnalytical Core
Test No.CA2023
Test Price$75.00 per assay
Description• For acetyl-CoA carboxylase, the tissue extract is incubated with unlabeled
acetyl-CoA + NaH13CO3 to form [13C]malonyl-CoA which, after hydrolysis, is
assayed as its TMS derivative.

[U-13C3]Malonyl-CoA is used as an internal standard.

• For malonyl-CoA decarboxylase, we developed two different assays. The first
assay involves incubation with [2-14C]malonyl-CoA, to form [2-14C]acetyl-CoA
which is reacted with carnitine to form [2-14C]acetyl-carnitine the
radioactivity of which is counted after isolation.

• The second assay involves incubation with [U-13C3]malonyl-CoA to form
[1,2-13C2]acetyl-CoA. The latter is reacted with thiophenol, and
acetylthiophenol is assayed by GC-MS, [2H3, 1-13C]acetyl-CoA is used as internal
standard.
DataNo data has been collected for this catalog item
Keywords

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Lipid Metabolism
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No phenotype assays found.
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