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Is apolipoprotein A-IV rate-limiting in the intestinal transport and absorption
of triglyceride?

Authors	Kohan AB, Wang F, Li X, Vandersall AE, Huesman S, Xu M, Yang Q, Lou D, Tso P
Submitted By	Patrick Tso on 5/13/2013
Status	Published
Journal	American journal of physiology. Gastrointestinal and liver physiology
Year	2013
Date Published	4/18/2013
Volume : Pages	304 : G1128 - G1135
PubMed Reference	23599044
Abstract	Apolipoprotein A-IV (apoA-IV) is synthesized by the intestine and secreted when dietary fat is absorbed and transported into lymph associated with chylomicrons. We have recently demonstrated that loss of apoA-IV increases chylomicron size and delays their clearance from the blood. There is still uncertainty, however, about the precise role apoA-IV on the transport of dietary fat from the intestine into the lymph. ApoA-IV KO mice do not have a gross defect in dietary lipid absorption, as measured by oral fat tolerance and fecal fat measurements. Here, using the in vivo lymph fistula mouse model, we show that the cumulative secretion of triglyceride (TG) into lymph in apoA-IV KO mice is very similar to that of WT mice. However, the apoA-IV KO mice do have subtle changes in TG accumulation in the intestinal mucosa during a 6 hour continuous, but not bolus, infusion of lipid. There are no changes in the ratio of esterified to free fatty acids in the intestinal mucosa of the apoA-IV KO, however. When we extended these findings, by giving a higher dose of lipid (6 µmole per h) and for a longer infusion period (8 hours), we found no effect of apoA-IV KO on intestinal TG absorption. This higher lipid infusion most certainly stresses the intestine, as we see a drastically lower absorption of TG (in both WT and KO mice), but the loss of A-IV does not exacerbate this effect. This supports our hypothesis that apoA-IV is not required for TG absorption in the intestine.

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