Real-time imaging of VCAM-1 mRNA in TNF-a activated retinal microvascular
endothelial cells using antisense hairpin-DNA functionalized gold nanoparticles.
Authors Uddin MI, Jayagopal A, Wong A, McCollum GW, Wright DW, Penn JS
Submitted By Ashwath Jayagopal on 9/13/2017
Status Published
Journal Nanomedicine : nanotechnology, biology, and medicine
Year 2017
Date Published 9/7/2017
Volume : Pages Not Specified : Not Specified
PubMed Reference 28890107
Abstract Vascular cell adhesion molecule 1 (VCAM-1) is an important inflammatory
biomarker correlating with retinal disease progression. Thus, detection of
VCAM-1 mRNA expression levels at an early disease stage could be an important
predictive biomarker to assess the risk of disease progression and monitoring
treatment response. We have developed VCAM-1 targeted antisense hairpin
DNA-functionalized gold nanoparticles (AS-VCAM-1 hAuNP) for the real time
detection of VCAM-1 mRNA expression levels in retinal endothelial cells. The
AS-VCAM-1 hAuNP fluorescence enhancement clearly visualized the TNF-a induced
cellular VCAM-1 mRNA levels with high signal to noise ratios compared to normal
serum treated cells. The scrambled hAuNP probes were minimally detectable under
same image acquisition conditions. Intracellular hAuNPs were detected using
transmission electron microscopy (TEM) analysis of the intact cells. In
addition, the AS-VCAM-1 hAuNP probes exhibited no acute toxicity to the retinal
microvascular endothelial cells as measured by live-dead assay.