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Catalog Item
Hyperinsulinemic Clamp (Hypoglycemic or Euglycemic) using stable isotopes
Common Name(s): Hyperinsulinemic-euglycemic clamp

CenterCase Western Reserve University
Center CoreMetabolic Core
Test No.CA2013
Test PriceINQUIRE
DescriptionThe euglycemic-hyperinsulinemic clamp is used to investigate insulin action and
glucose metabolism in the intact, conscious mouse. The use of different glucose
or glucose analog tracers with the option of using dual tracers (3-H or 14-C
labeled) allows the determination of tissue-specific glucose uptake in addition
to whole body glucose production and disposal. We can also use stable isotopes
for the glucose clamp studies
A chronic indwelling catheter is surgically implanted into the right jugular
vein of the mouse. After a minimum of 4 days recovery, the mice are fasted the
morning of the experiment. The 120 minute protocol begins with a
prime-continuous infusion of glucose tracer and a prime-continuous infusion of
insulin, at a rate dependant on the goals and conditions of the experiment.
Glucose is infused at a rate that maintains euglycemia and is a measure of
insulin sensitivity. Tissues can be collected at the conclusion of the clamp for
later assessment of tissue-specific glucose uptake as well as other biochemical
assays as desired.
DataNo data will be collected for this catalog item
Keywordsclamp, diabetes, glucose, glucose metabolism, hormone, insulin, insulin action,
insulin resistance, insulin secretion
Tissuespancreas

No catalog groups / subjects found.



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