Isotope Tracers in Metabolic Research
| Announcing an NIH/MMPC sponsored course: |
4th Annual Course on
Isotope Tracers in Metabolic Research:
Principles and Practice of Kinetic Analysis
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April 17-22, 2011
The Peabody Little Rock
Little Rock, Arkansas
Course Directors: Robert Wolfe, PhD and Henri Brunengraber*, MD, PhD
This is the fourth annual offering of a course that provides basic introductory and
comprehensive information on performing metabolic studies using tracers labeled with
radioactive or stable isotopes, in man and in animals. The course is designed for
beginners as well as those with experience who wish to expand their capabilities
to more sophisticated problems. The faculty is well-versed in a variety of applications
and methodologies. The number of students is limited to 100.
Techniques will be presented for investigating whole body metabolism, for metabolite
balance across organs, intracellular flux rates and pathway regulation. General aspects
of modeling will be considered, as well as specific applications in the study of
carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical
matters related to sample analysis by mass spectrometry and NMR will be discussed,
including detailed numerical examples of calculations involved in determining isotopic
enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail
the use of positional and mass isotopomer analysis for intracellular flux rates and
various aspects of protein and amino acid metabolism. Applications in humans and
animal models (particularly mouse) will be considered.
Course material will be presented in organized lectures and informal workshops. Problem
sets and discussion questions will highlight each lecture. Lecture notes and problem sets
will be available to registrants to be downloaded from the web at the course. In addition
to organized sessions, attendees will have ample opportunities for personal interaction
with faculty members to discuss issues of particular interest to them in depth.
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*supported by R25 DK082376 to H. Brunengraber
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Director & Faculty Information
| Faculty: |
| Henri Brunengraber -- Course Co-Director |
| Shawn C. Burgess |
| Gary Cline |
| Nicolaas E. Deutz |
| Joanne Kelleher |
| Craig Malloy |
| Owen McGuinness |
| Bettina Mittendorfer |
| Stephen Previs |
| Michelle Puchowicz |
| Robert Wolfe -- Course Director |
Sponsored by:
Grant R25-DK082376 from NIDDK to H. Brunengraber
Mouse Metabolic Phenotyping Centers
University of Arkansas for Medical Sciences Donald W. Reynolds Institute on Aging
The National Institute of Diabetes & Digestive & Kidney Diseases
The National Heart, Lung & Blood Institute
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Course Information
2010 Course Agenda:
Click here to download course syllabus.
Course Materials:
Materials will be provided online to be downloaded at the course. Participants should bring a laptop with wireless capability
for this purpose, and for homework problems. In addition, the following materials are highly recommended:
- “Isotope Tracers in Metabolic Research: Principle and Practice of Kinetic Analysis” by Robert R. Wolfe and David L. Chinkes, 2nd Edition, 2005. Wiley.
- Literature on NMR Isotopomer Analysis (citations to be provided)
- Two software programs, tcaSIM and tcaCALC, are available for download. To download the software, go to Download Registration and complete the
registration form. The tcaSim program is a tool for use with labeling studies in intermediary metabolism - primarily those applying 13C NMR spectroscopy,
but also for 14C radiolabel studies and 13C mass isotopomer analysis. The simulation has been developed to assist in the design of experiments and to help the
user understand the effect of various metabolic states and substrate enrichment levels on labeling patterns. The tcaCALC program performs an isotopomer analysis
to estimate relative pathway fluxes in biological systems at metabolic and isotopic steady state. It is used in 13C labeling studies where biological systems (isolated
cells, perfused organs or intact animals) are provided enriched substrate(s), and the labeling of intermediates detected using 13C NMR spectroscopy, mass- and tandem
mass spectrometry. The 13C spectra are collected either directly from intact tissue, such as in the case of perfusions conducted inside a magnet, or after extraction
of a tissue sample (isolated cell studies or tissue samples taken after in vivo infusion of 13C-enriched substrates).
Logistics:
Classes will be held at The Peabody Little Rock Hotel.
Please plan to arrive Sunday night, April 17, and to leave after noon on Friday, April 22. The airport is 15-20 minutes from the hotel.
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Registration & Accomodations
Registration and Payment must be received prior to March 31, 2011
Registration Fee:
Breakfast, lunch, 1 dinner, and course materials are included in the registration fee. Limited to 100 participants.
| Students / Post docs | $350 |
| Academic / Government scientists | $725 |
| Industry scientists | $1225 |
Registration Form:
Click here to download the Registration Form.
Checks for registration should be sent to:
Isotope Tracers Course
Deb Viane, Assistant
4301 W. Markham Street, Slot 806
Little Rock, AR 72205-7199
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Contact Information
Deborah Viane, Course Assistant
4301 W. Markham St., Slot 806
Little Rock, AR 72205-7199
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Other Upcoming Events
The MMPC is planning several small, hands-on interactive courses to teach students some of the technical skills
associated with isotope tracer kinetics and mouse metabolic phenotyping.
| Fall, 2009 | Mass Isotopomer Analysis (Mass Spectrometry) |
| Case Western Reserve University |
| Cleveland, Ohio |
| Contact information coming soon... | |
| Fall, 2010 | Positional Isotopomer Analysis (NMR Spectroscopy) |
| University of Texas Southwestern Medical Center |
| Dallas, Texas |
| Contact information coming soon... | |
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