Isotope Tracers in Metabolic Research
Announcing an NIH/MMPC sponsored course:
9th Annual Course on
Isotope Tracers in Metabolic Research:
Principles and Practice of Kinetic Analysis

November 7-11, 2016
Homewood Suites by Hilton® Nashville Vanderbilt
2400 West End Avenue
Nashville, Tennessee 37203
(also where the course takes place)

Course Co-Directors: Owen McGuinness, PhD, Henri Brunengraber, MD, PhD and Robert Wolfe, PhD

Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)

Welcome to the ninth annual course which provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes, in humans and in animals. The course is designed for beginners as well as those with experience who wish to expand their capabilities to more sophisticated problems. The faculty is well-versed in a variety of applications and methodologies. Techniques will be presented for investigating whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation. The basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. Applications in humans and animal models (particularly mouse) will be considered. Course material will be available for download from

Problems and discussion questions will highlight key concepts. In addition to organized sessions, individual attendees will have ample opportunities for personal interaction with faculty members in the form of one-on-one mentoring sessions to discuss their research projects in more depth.

Monday, November 7, 2016

Basic characteristics of radioactive, stable isotope tracers.
General principles of mass spectrometry.
Isotopic enrichment using GC-MS.
Methods of mass spectrometry analysis.
Measurement of specific activity.

Tuesday, November 8, 2016

Tracer kinetics (single pool models).
Oxidation and synthesis rates.
Glucose metabolism (clamp studies).
Lipid metabolism (basic kinetics).

Wednesday, November 9, 2016

Pathway fluxes using NMR isotopomer analysis.
Methods in protein metabolism.

Thursday, November 10, 2016

Energy expenditure with doubly labeled water.
Synthesis rates with deuterated water: proteins, fatty acids sterols, glucose, nucleic acids.
Mass isotopomer distribution analysis: polymer synthesis, multiple flux pathways, TCA cycle, anaplerosis.

Friday, November 11, 2016

Pathway discovery via association of isotopomer analysis and metabolomics.
Inherently difficult problems.

FOR COURSE DETAILS OR SUGGESTIONS: Amanda Zetans ( (615-343-1065)

Director & Faculty Information
Douglas E. BEFROY, DPhil.Kent, United Kingdom
Henri BRUNENGRABER, MD PhDCleveland, OH -- Course Co-Director
Gary CLINE, PhDNew Haven, CT
Melanie CREE GREEN, MD PhDDenver, CO
Joanne KELLEHER, PhDBoston, MA
Maren LAUGHLIN, PhDBethesda, MD
Owen McGUINNESS, PhDNashville, TN -- Course Co-Director
Matthew MERRITT, PhDDallas, TX
Elizabeth PARKS, PhDColumbia, MO
Stephen PREVIS, PhDRahway, NJ
Michelle PUCHOWICZ, PhDCleveland, OH
Robert R. WOLFE, PhDLittle Rock, AR -- Course Co-Director
Jamey YOUNG, PhDNashville, TN

Sponsored by:
This course is supported by the Mouse Metabolic Phenotyping Centers (by grant 5U24DK076169-09).

Course Information
2016 Course Syllabus:
Click here to download course syllabus.

  • Registration opens on June 1,2016
  • Registration is available online (Register click here) Registration is limited to 100 participants
  • Course registration deadline: Monday, October 10, 2016
  • Please refer to ‘Registration link’ for list of fees (no credit cards)
  • Registration fee includes:
    ~ breakfast, lunch and snacks (Nov 7 - 11)
  • Please bring a Wi-Fi enable device to download and view all course material.
  • NOTE: The following book is also very useful "Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis" by Robert R. Wolfe and David L. Chinkes, 2nd Edition (2005 Wiley-Liss).

To book a room at the discount rate, please call 1(615)320-7772 or go directly to the following link (Lodging information can be found here: and mention the identifier:
9th Annual Tracer Course + Dates of course

Reservations must be made on or before 10/10/2016