Isotope Tracers in Metabolic Research
Announcing an NIH/MMPC sponsored course:
8th Annual Course on
Isotope Tracers in Metabolic Research:
Principles and Practice of Kinetic Analysis

November 16-20, 2015
Wyndham Cleveland at PlayhouseSquare
1260 Euclid Avenue
Cleveland, Ohio

Course Directors: Robert Wolfe, PhD, Owen McGuinness, PhD, and Henri Brunengraber, MD, PhD

Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)

This is the 8th annual offering of a course that provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes, in human and in animals. The course is designed for beginners as well as those with experience who wish to expand their capabilities to more sophisticated problems. The faculty is well-versed in a variety of applications and methodologies.

Techniques will be presented for investigating whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation. The basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. Applications in humans and animal models (particularly mouse) will be considered.

Course material will be available for download from

Problems and discussion questions will highlight key concepts. In addition to organized sessions, individual attendees will have ample opportunities for personal interaction with faculty members in the form of one-on-one mentoring sessions to discuss their research projects in more depth.

Isotec-Sigma-Aldrich is generously sponsoring five registration fellowships for graduate students and postdocs. To apply for a fellowship, go to

Director & Faculty Information
Douglas E. BEFROY, DPhil.New Haven, CT
Henri BRUNENGRABER, MD PhDCleveland, OH -- Course Co-Director
Gary CLINE, PhDNew Haven, CT
Melanie CREE GREEN, MD PhDDenver, CO
Joanne KELLEHER, PhDBoston, MA
Maren LAUGHLIN, PhDBethesda, MD
Owen McGUINNESS, PhDNashville, TN
Matthew MERRITT, PhDDallas, TX
Elizabeth PARKS, PhDColumbia, MO
Stephen PREVIS, PhDRahway, NJ
Michelle PUCHOWICZ, PhDCleveland, OH
Robert R. WOLFE, PhDLittle Rock, AR -- Course Director

Sponsored by:
This course is supported by the Mouse Metabolic Phenotyping Centers (by grant 5U24DK076169-09).

Course Information
2015 Course Syllabus:
Click here to download course syllabus.

Course Materials:
Materials will be provided online to be downloaded at the course. Participants should bring a laptop with wireless capability for this purpose, and for homework problems. In addition, the following materials are highly recommended:

  • “Isotope Tracers in Metabolic Research: Principle and Practice of Kinetic Analysis” by Robert R. Wolfe and David L. Chinkes, 2nd Edition, 2005. Wiley.
  • Literature on NMR Isotopomer Analysis (citations to be provided)
  • Two software programs, tcaSIM and tcaCALC, are available for download. To download the software, go to Download Registration and complete the
    registration form. The tcaSim program is a tool for use with labeling studies in intermediary metabolism - primarily those applying 13C NMR spectroscopy, but also for 14C radiolabel studies and 13C mass isotopomer analysis. The simulation has been developed to assist in the design of experiments and to help the user understand the effect of various metabolic states and substrate enrichment levels on labeling patterns. The tcaCALC program performs an isotopomer analysis to estimate relative pathway fluxes in biological systems at metabolic and isotopic steady state. It is used in 13C labeling studies where biological systems (isolated cells, perfused organs or intact animals) are provided enriched substrate(s), and the labeling of intermediates detected using 13C NMR spectroscopy, mass- and tandem mass spectrometry. The 13C spectra are collected either directly from intact tissue, such as in the case of perfusions conducted inside a magnet, or after extraction of a tissue sample (isolated cell studies or tissue samples taken after in vivo infusion of 13C-enriched substrates).

Classes will be held at Wyndham Cleveland at PlayhouseSquare. Please plan to arrive Sunday night, November 15th, and to leave Friday afternoon on Friday, November 20th.
The Cleveland Hopkins Int’l Airport is only within 20 minutes from the Wyndham Hotel by public transit.

Registration & Accommodations
Registration Fee:
Registration fee includes: ~ breakfast, lunch and snacks (Nov 16 - 20). Limited to 100 participants.

Students / Post docs$450
Academic / Government scientists$800
Industry scientists$1,300

Wyndham Cleveland at PlayhouseSquare
Cleveland, Ohio
1 (877) 999-3223
Reservations Identifier: 8th Annual isotope Tracers Course

Registration Form:
Please click here to download the Registration Form.

Registration and Payment must be received prior to October 26, 2015

To Pay via check:
Please Make Check Payable To:
Case Western Reserve University
Ref.: 8th Annual MMPC Course

Mail To: Case Western Reserve University
Attn: Joan Schenkel
Department of Nutrition - School of Medicine
10900 Euclid Avenue - BRB932
Cleveland, OH 44106-4954 (USA)

Contact Information
Joan Schenkel
Department of Nutrition - School of Medicine
10900 Euclid Avenue - BRB932
Cleveland, OH 44106-4954