Isotope Tracers in Metabolic Research
Announcing an NIH/MMPC sponsored course:
6th Annual Course on
Isotope Tracers in Metabolic Research:
Principles and Practice of Kinetic Analysis
March 11-15, 2013
The Peabody Little Rock
Little Rock, Arkansas

Course Directors: Robert Wolfe, PhD and Henri Brunengraber*, MD, PhD

Sponsored by NIDDK and organized on behalf of the NIH-sponsored Mouse Metabolic Phenotyping Centers (MMPC)

This is the fifth annual offering of a course that provides basic introductory and comprehensive information on performing metabolic studies using tracers labeled with radioactive or stable isotopes, in man and in animals. The course is designed for beginners as well as those with experience who wish to expand their capabilities to more sophisticated problems. The faculty is well-versed in a variety of applications and methodologies. The number of attendees is limited to 100.

Techniques will be presented for investigating whole body metabolism, for metabolite balance across organs, intracellular flux rates and pathway regulation. General aspects of modeling will be considered, as well as specific applications in the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. Applications in humans and animal models (particularly mouse) will be considered.

Course material will be presented in organized lectures and informal workshops. Problem sets and discussion questions will highlight each lecture. Lecture notes and problem sets will be available to registrants to be downloaded from the web at the course. In addition to organized sessions, attendees will have ample opportunities for personal interaction with faculty members to discuss issues of particular interest to them in depth.

*supported by R25 DK082376 to H. Brunengraber

Isotec-Sigma-Aldrich is generously sponsoring five registration fellowships for graduate students and postdocs. To apply for a fellowship, go to

Director & Faculty Information
Douglas Befroy
Henri Brunengraber -- Course Co-Director
Gary Cline
Joanne Kelleher
Maren Laughlin
Owen McGuinness
Matthew Merritt
Elizabeth Parks
Stephen Previs
Michelle Puchowicz
Robert Wolfe -- Course Director

Sponsored by:
Grant R25-DK082376 from NIDDK to H. Brunengraber

Mouse Metabolic Phenotyping Centers
University of Arkansas for Medical Sciences Donald W. Reynolds Institute on Aging
The National Institute of Diabetes & Digestive & Kidney Diseases
The National Heart, Lung & Blood Institute

Registration & Accommodations
Registration and Payment must be received prior to March 1, 2013

Registration Fee:
Breakfast, lunch, 1 dinner, and course materials are included in the registration fee. Limited to 100 participants.

Students / Post docs$400
Academic / Government scientists$750
Industry scientists$1250

1-800-Peabody (1-800-732-2639)
Little Rock
Discounted rate: $145/night
Reservations Identifier: 6th Annual isotope Tracers Course

Registration Form:
Click here to download the Registration Form.
Please send registration form with your check to:
Isotope Tracers Course
Deb Viane, Assistant
4301 W. Markham Street, Slot 806
Little Rock, AR 72205-7199

Contact Information
Deborah Viane, Course Assistant
4301 W. Markham St., Slot 806
Little Rock, AR 72205-7199
Phone: 501-526-5709