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Strain
C57BL/6-Ldlrtm1Her Acvrl1tm2Spo Tg(Cdh5-cre/ERT2)1Rha

Summary Data Summary
Official Name C57BL/6-Ldlrtm1Her Acvrl1tm2Spo Tg(Cdh5-cre/ERT2)1Rha
Common Name endothelial specific het ALK1 on global LDLR KO
Description Ldlr knockout: Insertion of a neomycin resistance cassette
into exon 4. The authors predict that the targeted allele
would encode a truncated non-functional protein that will
not bind LDL, and that lacks a membrane spanning segment.
Immunoblot analysis of liver membranes detected a truncated
protein in homozygous mutant animals.

Acvrl1 knockin: A loxP site was inserted upstream of exon 4
and a floxed neo cassette was inserted downstream of exon 6.

Tg(Cdh5-cre/ERT2): The tamoxifen-inducible cre/ERT2 sequence
was inserted into a PAC (P1 artificial chromosome)
containing the VE-cadherin (Cdh5) gene to generate the
transgene construct.
Development Status Phenotyping ongoing
Creation Method knockout
TypeCount
Investigators 1
Genomics - Modifications 2
Genomics - Transgenes 1
Experiments 1


Investigators
NameInstitution
Monica LeeYale University


Genomic Information
GeneAllele 1Allele 2Protocol
LdlrknockoutknockoutNot Specified
Acvrl1knockin (lox)knockin (lox)Not Specified
EnhancerPromoterGeneCopies
Not SpecifiedCdh5CRENot Specified


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