D2001
Importation of Mice and Material
Assume 10 experimental mice {5M, 5F} and 10 WT controls {5M, 5F}
Importation of 1 crate of mice
$148.00 / $214.30 (University of California fee / all other institutions)
D2002
Conventional Per Diem
Cage/Day
$1.20 / $1.59 (University of California fee / all other institutions) per cage per day
D2003
Colony Management (if needed)
: A colony management fee is applied to projects requiring more than routine housing and care. This fee is applied on a per cage per day basis. Colony management includes (but is not limited to) projects requiring timed matings with or without plug collection; breeding with pup identification; collection of samples for genotyping; special diets.
$0.53 / $0.77 (University of California fee / all other institutions) per cage per day
D2004
Genotyping (if needed)
Some projects may require genotyping during the course of housing mice at UCD. Genotyping sampling may be done by ear punch or tail snips, depending on age. Altricial pups will be sampled by “toeing”. This fee is applied on a per sample basis, to samples submitted to the MBP Murine Genotyping Analysis laboratory for testing.
$13.00 / $18.82 (University of California fee / all other institutions) per sample
D2005
Mouse Model Purchase
Access mutant mouse models from the MMRRC or the KOMP Repositories and submit to MMPC for phenotyping
inquire
D2007
Chronic high-fat/high sugar diet feeding
An experimental mouse model of obesity can be generated by feeding either a high fat diet (HFD) or very high fat diet (VHFD) for specified period of time; diet based on investigator preference for study needs. Differences in body weight can be seen as soon as 2 weeks after the initiation of feeding HFD. After 4 weeks on HFD, many mice begin exhibiting hyperglycemia with the full obese phenotype measureable after 16 weeks. Within 16-20 weeks mice may experience a 20-30% increase in body weight, adipocyte hyperplasia, fat deposition in the mesentery, increased fat mass, diabetes, and hypertension. The effect of the diet on obesity development is directly related to the fat content of the diet and the length of time the animal spends on the diet with variability in severity of metabolic changes observed between individual animals. The Animal Care Core works with the users in designing a study with appropriate feeding duration and selection of diet with respect to percent and composition of fat in the diet.
Inquire – Contact UCD Animal Care Core
D2014
Basic Mouse Necropsy
Diagnostic basic gross necropsy examination of your mouse model. Services include anatomic pathology, tissue characterizations and staining, diagnostic histological examination. Upon request, we also will perform tissue collection, serum/plasma/urine chemistry, hematology analysis, and urinalysis. These will be completed by a highly trained lab technician.
$75.00
D2015
Advanced Mouse Necropsy
Diagnostic advanced gross necropsy examination of your mouse model. Services include anatomic pathology, tissue characterizations and staining, diagnostic histological examination. Upon request, we also will perform tissue collection, serum/plasma/urine chemistry, hematology analysis, and urinalysis. These will be completed by a highly trained lab technician.
$146.00
D2016
Additional Pathology Services
The scope of Additional Pathology Services can encompass a wide variety of activities that can enhance routine histopathology analysis and processing.
Special tissue collections requiring special expertise, time and labor-intensive processing and analyses could be included in "Additional Pathology Services". Creation and processing of gut-rolls or special organ preparations, precise brain sectioning and processing, and a myriad of advanced photo-microscopy or "Count & Measure" analyses are possible. We have imaging analysis software that allows Pixel-Dependent reviews and analysis of stained tissues.
Inquire please. Additional services costs are dependent on technologist or pathologist technical time, special materials or special instrumentation requirements.
D2017
Surgical Sevices
Surgical services including bariatric surgery (Vertical Sleeve Gastrectomy or Roux en Y) ,telemeter, data recorder or osmotic pump implantation etc.
Inquire please
D2018
Barrier per diem
Housing 1 – 4 mice per cage/day
$2.11
D2019
Blood Collection
Submandibular, Saphenousvein, Processing, per mouse
$25.00
D2020
Food/Water Intake
per mouse
$10.00
D2021
Body Weight
per mouse
$6.00
D2022
DVM Services
per hour
$90.00
D3001
IN VIVO Insulin Tolerance Tests
Mice will be injected IP with 1mU/g of insulin. Samples will be collected at 0,15,30,45,60,90,120 min for the measurement of glucose.Plumpton,1969
Includes housing, surgery, biochemical assays to measure glucose/insulin level.
Mice from an inbred strain with low inter-animal variability will be run with each group of animals undergoing the same procedure
$96.72 / $129.31(Internal University of California Fee / All Other Institutions)
D3002
IN VIVO Glucose Tolerance Tests (IP or Oral)
Mice will be injected IP with 2mg/g of glucose. Samples will be collected at 0,15,30,60,120 min for the measurement of glucose.
Includes housing, surgery, biochemical assays to measure glucose/insulin level.
Mice from an inbred strain with low inter-animal variabilitywill be run with each group of animals undergoing the same procedure.
$122.31
D3003
IN VIVO Glucose-stimulates Insulin Secretion Test
Mice will be injected IP with 2mg/g of glucose. Samples will be collected at 0,2,5,15,30 min for the measurement of glucose and insulin.
Includes housing, surgery, biochemical assays to measure glucose/insulin level.
Mice from an inbred strain with low inter-animal variabilitywill be run with each group of animals undergoing the same procedure
$172.41
D3101
Lipid extraction from liver
Triglyceride and cholesterol content in liver tissue will be determined by the Folch method (Folch, 1957). Weighed tissue samples are homogenized in methanol:chloroform. After overnight extraction, 0.7% sodium chloride is added. The aqueous layer is aspirated and duplicate aliquots of the chloroform/lipid layer are dried under nitrogen gas. The lipid is reconstituted in isopropyl alcohol and assayed for TG spectrophotometrically with enzymatic reagents from Fisher Diagnostics (Middletown, VA)
inquire
D3102
Lipid extraction from muscle
Triglyceride and cholesterol content in muscle tissue will be determined by the Folch method (Folch, 1957). Weighed tissue samples are homogenized in methanol:chloroform. After overnight extraction, 0.7% sodium chloride is added. The aqueous layer is aspirated and duplicate aliquots of the chloroform/lipid layer are dried under nitrogen gas. The lipid is reconstituted in isopropyl alcohol and assayed for TG spectrophotometrically with enzymatic reagents from Fisher Diagnostics (Middletown, VA)
inquire
D3103
Glucose (urine/plasma)
Hyperglycemia/ glycosuria
Thermo microplate (5ul) enzymatic assay
inquire
D3104
Hemoglobin A1C
Index of long-term glycemic control.
Diazyme (20ul whole blood) enzymatic assay.
inquire
D3105
beta-hydoxybutyrate
Markers of ketosis.
Millipore-Sigma enzymatic assay (20ul for duplicates).
inquire
D3106
Triglyceride
This assay uses reagents from Millipore-Sigma and 10ul of sample is required for duplicates, an additional 5ul of sample is recommended for a sample blank if the sample is hemolyzed.
inquire
D3107
Total Cholesterol
This assay uses reagents from Fisher Diagnostics and 10ul of sample is required for duplicates, an additional 5ul of sample is recommended for a sample blank if the sample is hemolyzed.
inquire
D3108
HDL-C and LDL-C/VLDL-C
This assay uses a precipitation buffer from AbCam to precipitate LDL and VLDL particles and 25ul of sample is required. The cholesterol content will be determined in the HDL fraction, and this value will be subtracted from the total cholesterol of the original sample to provide the cholesterol value for the LDL/VLDL fraction.
inquire
D3109
HDL-TG and LDL-TG/VLDL-TG
This assay uses a precipitation buffer from AbCam to precipitate LDL and VLDL particles and 25ul of sample is required. The triglyceride content will be determined in the HDL fraction, and this value will be subtracted from the total triglyceride of the original sample to provide the triglyceridel value for the LDL/VLDL fraction.
inquire
D3110
Non-esterified fatty acids
WAKO microplate (10ul for duplicates) with standard reagents from PolyMedCo
inquire
D3111
Metabolomics
Multiple metabolite levels measured. Intensity values normalized to total metabolome content. Gass Chromatograph (GC)-Time of Flight (TOF) mass spectrometry. MS deconvolution, BinBase DB processing. compounds.
inquire
D3112
Complex lipid ratios
Multiple metabolite levels measured. Intensity values normalized to total metabolome content. UPLC/QTOF mass spectrometry. MZmine data processing. Identification on accurate mass and MS/MS databases.
inquire
D3113
Hormones - Generic ELISA Assay
Any hormone can be measured if it is offered in an assay format without extraction (for example IGF1, Corticosterone, Resistin, etc).
inquire
D3114
Insulin
Fasting insulin is an index of IR. Also measured for functional tests. Meso Scale Discovery (10ul for duplicates)
inquire
D3115
C-Peptide
Proinsulin cleavage product. ALPCO ELISA (10ul)
inquire
D3116
Leptin
Adipocyte hormone involved in energy balance/anti-steatotic Meso Scale Discovery (10ul for duplicates)
inquire
D3117
Adiponectin (total)
Insulin-sensitizing/anti-steatotic/anti-atherogenic adipocyte hormone
Meso Scale Discovery (5ul)
inquire
D3118
Adiponectin (HMW)
Insulin-sensitizing/anti-steatotic/anti-atherogenic adipocyte hormone. ALPCO ELISA (5ul)
inquire
D3119
Glucagon
Counterregulatory hormone. Millipore RIA (100ul)
inquire
D3120
Glucagon-like peptide 1 (active)
Incretin hormone. Meso Scale Discovery (20ul for duplicates)
inquire
D3121
Glucagon-like peptide 1 (total)
Incretin hormone. Meso Scale Discovery (20ul for duplicates)
inquire
D3122
Ghrelin
Orexigenic GI hormone. Millipore ELISA (50ul)
inquire
D3123
Markers of Inflammation - custom panel
Inflammation plays a crucial role in atherosclerosis and contributes to insulin resistance. Up to 10 proinflammatory cytokines (IFN-?, IL-1ß, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 p70, KC/GRO, TNF-a) can be measured with 25µl of serum (50µl for duplicates) using a Meso Scale Discovery Sector Imager.
inquire
D3124
HS CRP
Associated with insulin resistance and dyslipidemia. R&D Systems ELISA (10ul)
inquire
D3125
Corticosterone
10ul of serum or EDTA Plasma measured by RIA (RadioImmuno Assay)
inquire
D3127
Lipopolysaccharide Binding Protein (LBP)/Endotoxemia Assay
Plasma samples will be assayed for lipopolysaccharide binding protein (LBP) as surrogate for bacterial LPS/measure of endotoxemia via ELISA.
$14.00
D3128
Fecal Mouse Lipocalin-2
Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), 2 is a secreted glycoprotein that can be used as a biomarker of inflammation. Lipocalin-2 is expressed in intestinal epithelium and is upregulated in models of intestinal inflammation and injury. Fecal lipocalin-2 levels correlate well to other markers of intestinal inflammation. Lipocalin-2 will be measured by ELISA.
inquire
D3129
Fecal Mouse Calprotectin
Calprotectin is a heterodimer of calcium binding proteins S100A8 and S100A9. Calprotectin is almost exclusively expressed in neutrophils and precursors cells in the bone marrow. Fecal calprotectin levels can be used as a marker for intestinal inflammation, reflecting the recruitment and activation of neutrophils to the mucosa. Fecal calprotectin is often used clinically to diagnose inflammatory bowel diseases and correlate well to other well-established clinical markers of intestinal inflammation. Fecal homogenates will be assayed for S100A8/S100A9 heterodimer by ELISA.
inquire
D3130
Plasma/Serum Mouse Lipocalin-2
Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), 2 is a secreted glycoprotein that can be used as a biomarker of inflammation. Lipocalin-2 is increased in metabolic diseases associated with an inflammatory phenotype, including type 2 diabetes, obesity and liver steatosis. Lipocalin-2 is highly expressed in white adipose tissue and tracks obesity. Strong correlations between blood concentrations of lipocalin-2 and insulin resistance and type 2 diabetes have been noted. Lipocalin-2 can be measured in urine, blood, and feces. Lipocalin-2 will be measured by ELISA.
inquire
D3131
Plasma/Serum Mouse Calprotectin
Calprotectin is a heterodimer of calcium binding proteins S100A8 and S100A9. Calprotectin is almost exclusively expressed in neutrophils and precursors cells in the bone marrow. Plasma/serum calprotectin levels can be used to monitor diseases involving chronic inflammation such as rheumatoid arthritis, inflammatory bowel diseases, and systemic lupus erythematosus. Calprotectin levels often correlate with obesity and severity of metabolic disease. Plasma/Serum will be assayed for S100A8/S100A9 heterodimer by ELISA.
inquire
D3132
Hepatic Triglyceride production
Measures temporal TG production under conditions in which TG hydrolysis by lipoprotein lipase (LPL) is inhibited with nonionic detergent (i.e., Triton WR-1339 or poloxamer 407).
inquire
D3201
Exercise capacity
Treadmill test for forced and tolerance exercise tests.
inquire
D3202
Gross Body Composition- Echo MRI
Measures adiposity, lean mass using Echo-MRI
inquire
D3203
Adiposity (adipose depot weights)
Measures adipose tissue weights, by manual tissue dissection.
$63.00
D3204
Energy Expenditure (CLAMS, Indirect Calorimetry)
Measures energy utilization and fuel preference by indirect calorimetry; gross 24 hour food and water intake; body composition by Echo-MRI
$68.00
D3205
Meal Pattern Analysis
Detailed analysis of food intake behavior including Avg Meal Duration-Active Eating Time (sec), Avg Meal Size (g), # of Meals, Avg Inter-Meal Interval (IMI) (sec), Total Meal Duration, Satiety Ratio, Avg Eating Rate, Avg Light-Meal Duration, Avg Dark-Meal Duration, Avg Light-Meal Size, Avg Dark-Meal Size, Avg Light-IMI, Avg Dark-IMI, Light- # Meals, Dark- # Meals, Light-Satiety Ratio, Dark-Satiety Ratio, Light-Eating Rate (g/min), Dark-Eating Rate (g/min), Food Intake/BW). "Meal" defined by minimum food intake of 0.02g and at least 10 min between food bout events.
$52.00
D3206
Energy Expenditure (CLAMS, Indirect Calorimetry) + Meal Pattern Analysis
Measures energy utilization and fuel preference by indirect calorimetry; gross
24 hour food and water intake; body composition by Echo-MRI
-and-
Detailed analysis of food intake behavior including Avg Meal Duration-Active
Eating Time (sec), Avg Meal Size (g), # of Meals, Avg Inter-Meal Interval (IMI)
(sec), Total Meal Duration, Satiety Ratio, Avg Eating Rate, Avg Light-Meal
Duration, Avg Dark-Meal Duration, Avg Light-Meal Size, Avg Dark-Meal Size, Avg
Light-IMI, Avg Dark-IMI, Light- # Meals, Dark- # Meals, Light-Satiety Ratio,
Dark-Satiety Ratio, Light-Eating Rate (g/min), Dark-Eating Rate (g/min), Food
Intake/BW). "Meal" defined by minimum food intake of 0.02g and at least 10 min
between food bout events.
$110.00
D3207
Gut Microbiome Analysis
Determines differences in gut microbial community and diversity; 16S rDNA sequencing Ion PGM- 20000 avg read depth
$276.00
D3208
Xenometabolomics analysis
The metabolites that gut bacteria produce are important to regulation of gut health, inflammation, and metabolism.
$276.00
D3209
Digestible Energy by Bomb Calorimetry
Assesses efficiency of gut energy uptake; bomb calorimetry of fecal samples and diet.
$132.00
D3210
Metabolic & Feeding Hormone Responsiveness
Measure changes in diet intake in response to peptide hormones (CCK, Leptin, etc.)
inquire
D3211
Diet Preference Test
Measure changes in diet preferences using the BioDAQ system.
inquire
D3301
CT, MRI, PET, & combinations
CT, MRI, PET, & combinations. Cardiac or brain are assessed.
http://imaging.bme.ucdavis.edu/faqs/recharge-rates/
inquire
D3302
Positron emission tomography (microPET)
Non-invasive longitudinal analysis of changes in glucose metabolism. Mice from an inbred strain with low inter-animal variabilitywill be run with each group of animals undergoing the same procedure.
inquire
D3303
Whole body MRI adipose depots
Adipose depot-specific lipid quantification by segmentation of whole body MRI images
inquire
D3304
Tissue-specific MRI
Lipid quantification by segmentation of whole body MRI images
inquire
D3401
Special Services
Consultation; Study design or specialized data analysis. (Advanced data analysis or data analyses from data obtained outside core.)
$91.00
D4001
Clinical Chemistry, Hematology, Urine Analysis
A wide variety of clinical chemistry and hematology assays are available for characterization of rodent blood and rodent strain types. The Comparative Pathology Laboratory has special instrumentation that can analyze extremely small sample volumes. We are able to provide analytical services to many types of clients, but we are optimized for rodent researchers. Please inquire for a consultation.
inquire
D4002
Urinary Albumin Excretion
Index of renal damage/Impaired glomerular function. 24 hour urine samples are collected from animals in a metabolic cage and albmunin is measured with a standard assay kit, using Albumin Blue 580 Fluorescence
$14.71
D4003
Glomerular Filtration Rate
Noninvasive GFR (FITC-insulin test)
inquire
D4004
Spot urine collection
Collection of urine sample
inquire
D4101
Eye Exam
Non-invasive evaluation of anterior/posterior eye chambers
$45.00
D4102
Voluntary wheel running (activity and/or exercise)
Voluntary wheel running provides detailed information on the running capacity and activity patterns (i.e., total distance, median velocity, time of breaks, etc.). This system may also be used as a means of providing voluntary exercise in metabolic or exercise based studies.
Per mouse/week fee (minimum of 8 mice): $14 / $19 (Internal University of California Fee / All Other Institutions)
D4103
Core body temperature (temperature recorder)
Measures core body temperature with IP temperature recorder (DST-nano-T). This service is paired with Data Recorder Implant Surgery through the animal core.
Per mouse/day use fee: $6 / $8 (Internal University of California Fee / All Other Institutions)
D4201
BP measurement by tail cuff
BP measurement by tail cuff. The technique provides a good estimate of actual systolic pressure.
$30.00
D4202
Echocardiography
Echocardiography. Cardiac chamber dimensions, valvular abnormalities, & cardiac output.
inquire
D4203
Cardiac electrophysiology
Cardiac electrophysiology. Baseline & after provocation will be assessed.
$192 / $256 (Internal University of California Fee / All Other Institutions) per mouse
D4301
Ex vivo assessment of barrier function/gut permeability (minimum of 8 samples)
Gut tissue (by region- e.g ileum & colon) will be opened along the mesenteric border and mounted in Ussing chambers (Physiologic Instruments, San Diego, CA, USA), exposing 0.3 cm2 of tissue surface area to 2.5ml of oxygenated Krebs-glucose (10mM) and Krebs-mannitol (10mM) at 37°C on the serosal and luminal sides, respectively. The paracellular pathway and transcellular pathway will be measured as the flux of FITC-Dextran 4000 (FD-4, Sigma –Aldrich) and horseradish peroxidase (HRP Type VI, Sigma Aldrich), respectively. FD-4 (400µg/ml) and HRP (200µg/ml) will be added to the mucosal chamber and samples will be collected from the serosal chamber every 30 min for 2 hours. Concentration of FD-4 is measured via fluorescence at excitation 485 nm, emission 538 nm. O-dianisidine substrate is used to detect HRP at absorbance 450 nm.
Per sample fee (minimum of 8 samples): $7/$10 (Internal University of California Fee / All Other Institutions)
D4302
Whole Intestinal transit time
Whole intestinal transit time
$105.00
D4303
Gut Permeability in vivo
Evaluation of intestinal transport
$185.00
D4304
Fecal collection
Collection of feces
inquire
D4401
Cognitive Function - Radial Arm Water Maze
This is a spatial learning and memory task. Animals are tested for 9 daily sessions of five trials. Each session consists of four learning trails and a fifth retention trial 30 minutes after trial four over a ten consecutive day period. Parameters such as error rate and latency aid in detecting cognitive deficits. Fees are quoted for a cohort of 8 mice.
inquire
D4402
Cognitive Function - Morris Water Maze (Male Mice)
The Morris water maze (MWM) is a widely used tool to study spatial learning and memory. It can assess learning, memory, and spatial working memory and can also indicative of damage to cortical regions of the brain. It can measure the effect of neurocognitive disorders on spatial learning and possible neural treatments, to test the effect of lesions to the brain in areas focused on memory, and to study how age influences cognitive function and spatial learning. The fee listed applies to a cohort of 8 mice.
inquire
D4403
Cognitive Function - Morris Water Maze (Female Mice)
The Morris water maze (MWM) is a widely used tool to study spatial learning and memory. It can assess learning, memory, and spatial working memory and can also indicative of damage to cortical regions of the brain. It can measure the effect of neurocognitive disorders on spatial learning and possible neural treatments, to test the effect of lesions to the brain in areas focused on memory, and to study how age influences cognitive function and spatial learning. Fees apply to a cohort of 8 Female mice.
inquire
D4404
Cognitive Function - Y Maze
The y-maze is a modification of the T- maze which evaluates memory and special learning in rodents though quantification of spontaneous alternation (a measure of spatial cognition). This measure has previously been shown to be affected in mouse models of Alzheimer’s. Fee is per mouse, 8 mouse minimum.
inquire
D4405
Light-Dark assay
Anxiety-like assay based on conflict
Inquire
D4406
3-chambered social approach
Choice of social stimulus versus novel object
Inquire
D4407
Fear Conditioning & Testing
Contextual and Cued learning and memory (3 day assay)
$88.00
D4408
Passive Avoidance
Aversion-dependent learning & memory test (2-day assay)
$88.00
D4409
Grip Strength
Forelimb & hindlimb grip force
$7.00
D4410
Startle & Prepulse Inhibition of Startle
Sensorimotor gating
$20.00
D4411
Novel Object/Location Recognition
Learning and memory (short or long term)
$37.00
D4412
Social Interaction
Dyad reciprocal social interactions
$37.00
D4413
Male-female social interaction
Male ultrasonic calling
$25.00
D4414
Marbles Burying
Repetitive Behaviors
$20.00
D4415
Touchscreen Operant Testing
Learning, Memory & Discrimination
$175.00
D4416
Fcn’l Observational Battery
Health, Morphology & Neurological assessments
$25.00
D4417
DigiGait
Gait evaluation
$37.00
D4418
Rotarod
Balance, Motor & Motor learning
$37.00
D4419
Open Field
Locomotion, anxiety
$20.00
D4420
Elevated plus-maze
Anxiety-like assay based on conflict
$20.00
D4421
Nociception
Thermal (Hargreaves) & Mechanical (von Frey) tests
Inquire
D4501
Wound healing
In vivo imaging of wound healing w. reporter mouse
Inquire
D4601
Special Services
Consultation; Study design or data analysis. (Advanced data analysis or data analyses from data obtained outside core.)
$68.00
MI1001
Free Study Design and Consultation
Free Study Design and Consultation with no need to inquire any studies
Free
MI1002
Chronic Catheterization of Jugular Vein
Catheterization of jugular vein
$78.37/mouse/procedure; $87.35/rat/procedure
MI1003
Chronic Catheterization of carotid artery
Catheterization of carotid artery
$100.55/mouse/procedure; $114.22/rat/procedure
MI1004
Catheterization of jugular vein AND carotid artery
Catheterization of jugular vein AND carotid artery
$168.67/mouse/procedure; $191.55/rat/procedure
MI1005
Catheterization of portal vein
Catheterization of portal vein
$127.04/mouse /procedure; $137.35/rat/procedure
MI1006
Catheterization of portal vein AND carotid artery
Catheterization of portal vein AND carotid artery
$165.0/animal/procedure
MI1007
Catheterization of stomach or bladder
Catheterization of stomach, intestine or bladder
$54.96/mouse/procedure; $70.32/rat/procedure
MI1008
Partial pancreatectomy
Partial pancreatectomy
$39.13/animal for 60% removal; $48.92/animal for 90% removal
MI1009
Parabiosis (a surgical union of two mice)
Parabiosis (a surgical union of two mice)
$151.37/procedure; $104.17 to include the reverse surgery
MI1010
Intracranial cannulation
Intracranial cannulation
$146.70/animal/procedure
MI1011
Renal capsual implantation
Renal capsual implantation
$48.98/animal/procedure
MI1012
Sub-total nephrectomy
Sub-total nephrectomy with a removal of up to 5/6 of total kidney
$136.85/mouse/procedure
MI1013
Glucose Tolerance Test (OGTT or IPGTT) including blood glucose and plasma insulin
Glucose Tolerance Test (OGTT or IPGTT) including blood glucose and plasma insulin
$80.12/mouse/procedure; $99.15/rat/procedure
MI1014
Glucose Tolerance Test (OGTT or IPGTT) w/o plasma insulin
Glucose Tolerance Test (OGTT or IPGTT) w/o plasma insulin
$50.64/mouse/procedure; $66.49/rat/procedure
MI1015
Insulin or Pyruvate Tolerance Test
Insulin or Pyruvate Tolerance Test
$44.29/mouse; $50.64/rat/procedure
MI1016
Hyperinsulinemic-euglycemic clamp (3H-glucose + 14C-2DG)
Hyperinsulinemic-euglycemic clamp (3H-glucose + 14C-2DG)
$491.89/animal
MI1019
Hepatic de novo lipogenesis during Hyperinsulinemic-euglycemic clamp (3H-glucose)
Hepatic de novo lipogenesis during Hyperinsulinemic-euglycemic clamp (3H-glucose)
$37.20/animal
MI1020
Hepatic glycogen synthesis
Hepatic glycogen synthesis during Hyperinsulinemic-euglycemic clamp (3H-glucose)
$37.20/animal
MI1022
Hyperglycemic clamp excluding plasma insulin ($10/sample)
Hyperglycemic clamp with blood glucose clamped at 300 mg/dL or double the baseline. Plasma insulin will be measured by ELISA and charged separately at $10/sample.
$422.76/mouse; $478.70/rat
MI1023
Hyperglycemic clamp
Hyperglycemic clamp
$422.76/mouse; $478.70/rat
MI1024
Whole-body energy expenditure for conventional or germ-free mice. Free body composition measurement.
Whole-body energy expenditure for conventional or germ-free mice. Free body composition measurement.
$95.00/3d, or more days @$30.00/d
MI1026
Whole-body energy expenditure + 13CO2 production (escluding compound cost)
Whole-body energy expenditure with instant measurement of 13CO2 production using 13C-labeled metabolic compound (separate cost)
$95.00/3d, or more days @$30.00/d; $1.99/d for 13CO2 production
MI1027
Non-shivering thermogenesis using injection of norepinephrine (including 24hours acclimation at 30°C)
Non-shivering thermogenesis using injection of norepinephrine (including 24hours acclimation at 30°C)
$55.11/mouse/visit
MI1028
Body Composition (EchoMRI 4in1-500)
Body Composition (EchoMRI 4in1-500)
$11.83/mouse/visit
MI1029
Organ or tissue composition
For freshly collected organ/tissue or after heated @ 37 °C
$3.87/ea
MI1030
Bomb calorimetry (Parr 6200, 1108 oxygen bomb) for measuring calorie content in food, feces, or tissue.
Samples will be dried, powdered, and mixed with unbleached wheat flour or benzuic acid at 1:9 by weight to form a 1.00g pellet. Each sample will be measured in duplicate.
$34.40/sample
MI1031
Fat Tolerance Test, Oral gavage (olive or corn oil)
Fat Tolerance Test, Oral gavage (olive or corn oil)
$54.19/mouse
MI1032
Fat fluxes (fat uptake and oxidation in tissues using 3H-triolene)
Fat fluxes (fat uptake and oxidation in tissue using 3H-triolene)
$140.63/animal
MI1033
Hepatic TG Secretion (Poloxamer 407 or Triton WR-1339)
Hepatic TG Secretion (Poloxamer 407 or Triton WR-1339)
$54.19/animal UofM fee (please inquire for external pricing)
MI1034
Automated Blood Sampling and/or Intravenous Infusion (Culex System) in conscious, free-moving and undisturbed mice (requiring surgical cannulation)
Automated Blood Sampling and/or Intravenous Infusion (Culex System), with NO human presence, sampling frewency goes up to every 5 minutes and a duration up to 7 days.
$185.44/mouse or $204.44/rat
MI1036
Steady-state isotopic labeling of metabolites for studying metabolomics (requiring surgical cannulation)
Steady-state isotopic labeling of metabolites (requiring surgical cannulation)
$223.30/mouse or $243.57/rat
MI1037
Steady-state isotopic labeling of metabolites under insulin clamp
Steady-state stable isotopic labeling of metabolites under insulin clamp: a combination of stable isotopic labeling and insulin clamp.
Please Inquire
MI1038
Treadmil Exercise Training
Treadmil running capacity training with various protocols.
$35.12/mouse/week
MI1039
Running Capacity (running time and distance to exhaustion)
Running Capacity (running time and distance to exhaustion)
$36.59/mouse/procedure
MI1040
Running Capacity (VO2max)
Running Capacity (VO2max): using Promethion or CLAMS @ a continuous VO2 and VCO2 sampling per 1-5 seconds and VO2max is defined as average of the highest 30sec values near to or at exhaustion.
$64.75/mouse/procedure
MI1041
In-cage Running Wheel Activity (Starr Life Sci) @RT
Cage size: 14.375"x8.125"x5,5" (LxWxH), with a virtical mounted stainless steel running wheel (w/H/Ø: 2"x4.75"x4.5" ), using Vitalview software to track wheel revlutions and running distance and time.
$2.49/mouse/day
MI1043
Manual Body Weight Recording
Manual Body Weight Recording
$2.82/mouse/visit; $3.26/rat/visit
MI1044
Manual Food or Water Recording
Manual Food or Water Recording
$3.33/mouse/visit; $3.83/rat/visit
MI1045
Oral Gavage
A commonly used medical process involving the insertion of a plastic tube through the mouth, past the throat, and down into the stomach.
$5.50/mouse/visit; $6.47/rat/visit
MI1046
Bronchoalveiolar Lavage
A medical process using a saline solution to wash the airways and capture a fluid sample at tissue dissection.
$13.23/animal/visit
MI1047
Rectal Temperature Recording (YSI 4600 precision thermometer)
Rectal Temperature Recording (YSI 4600 precision thermometer)
$3.98/mouse/visit; $4.48/rat/visit
MI1048
Body Temperature (subq, BAT, or IP) (requiring implantation of BMDS transponder)
The transponder chip (IPTT-300) will be surgically implanted in sub-cu or IP under light isoflurane anesthesia. Body temperature will be recorded using DAS7007s scanner without touching animals, Bio Medic Data Systems)
$4.02/mouse/visit; $4.55/rat/visit
MI1049
24-hour Urine and/or Fecal Collection
24-hour Urine and/or Fecal Collection
$9.46/mouse/day
MI1050
Tissue Dissection/Collection (Mouse)
Tissue Dissection/Collection (under pentobarbital/ketamine/isoflurane anesthesia or immediately after decapitation/cervical dislocation; tissues are immediately frozen in liquid N2, stored at -80°C, and delivered with dry ice)
$15.26/mouse + $3.40/sample and $5.62/brain sample.
MI1051
Tissue Dissection/Collection (Rat)
Tissue Dissection/Collection (under pentobarbital/ketamine/isoflurane anesthesia or immediately after decapitation/cervical dislocation; tissues are immediately frozen in liquid N2, stored at -80°C, and delivered with dry ice)
$25.54/rat + $3.95/sample and $6.37/brain sample
MI1052
Tail Vein Blood Sampling (plasma or serum)
Tail Vein Blood Sampling (plasma or serum)
$3.97/animal/visit, $4.08/animal/visit (heparin), $4.22/animal/visit (EDTA), or $4.97/animal/visit (serum)
MI1053
Retro-orbital bleeding (under isoflurane anesthesia)
Retro-orbital bleeding (under isoflurane anesthesia)
$1.80/sample for EDTA plasma extraction (using EDTA coated micro tube)
$2.60/sample for blood glucose (glucometer),
$2.50/sample for plasma glu
$5.87/animal/visit, $5.98/animal/visit (heparin), $6.20/animal/visit (EDTA), or $7.11/animal/visit (serum)
MI1054
Tail Vein Injection
Tail Vein Injection
$13.54/mouse/visit; $15.97/rat/visit
MI1055
IP Injection
Intraperitoneal injection or IP injection is the injection of a substance into the peritoneum (body cavity)
$2.91/mouse/visit; $3.31/rat/visit
MI1056
Subcutaneous Injection
Subcutaneous Injection
$2.71/mouse/visit; $3.11/rat/visit
MI1057
Plasma glucose (using a colorimetric kit)
Plasma glucose (using a colorimetric kit)
$3.27/sample
MI1058
Plasma insulin (using a ELISA kit)
Plasma insulin (using a ELISA kit)
$14.21/sample
MI1059
Plasma NEFA (using a colorimetric kit)
Plasma non-esterified fatty acids (NEFA) (using a colorimetric kit)
$6.68/sample
MI1060
Plasma TG (using a colorimetric kit)
Plasma triglyceride (TG) (using a colorimetric kit)
$6.68/sample
MI1063
Novel Objective Recognition Test (NORT)
NORT: a widely-used behavioral test for studying learning and memory in mice or rats. EthoVision XT by Noldus was used to record, track, and analyze all videos.
$34.32/animal
MI1064
Morris Water Maze
The Morris water maze is a behavioral procedure widely used in behavioral neuroscience to study spatial learning and memory. It enables learning, memory, and spatial working to be studied with great accuracy and can also be used to assess damage to particular cortical regions of the brain.
$124.11/mouse
MI1065
Open Field Test (OFT)
OFT: a commonly used test for measuring exploratory behavior, general locomotor activity, and anxiety-related emotional behaviors. EthoVision XT by Noldus was used to record, track, and analyze all videos.
$34.32/animal
MI1066
Light-Dark Box (LDB)
LDB: a widely-used behavioral test for studying anxiety in mice or rats. EthoVision XT by Noldus was used to record, track, and analyze all videos.
$34.32/animal
MI1067
Elevated Plus Maze (Ethovision)
The elevated plus maze is a well-characterized behavioral paradigm, one of the most used tests for anxiety research. The maze contains two open arms and two closed (wall-sheltered) arms and relies upon the animal’s natural tendency to stay in enclosed spaces and their unconditioned fear for open spaces and heights. In short, anxious animals will spend more time in the closed arms than less anxious animals.
$34.32/animal
MI1068
Y-maze
Y-maze: a frequently used test for assessing short term memory in mice. EthoVision XT by Noldus was used to record, track, and analyze all videos.
$34.32/animal
MI1069
Forced Swim Test
This task is used to assess depressive-like behavior (or a form of learned helplessness). Animals that are considered more depressive tend to give up sooner and more completely when faced with a high degree of adversity.
$34.32/animal
MI1070
Tail Suspension Test
This task is used to assess depressive-like behavior (or a form of learned helplessness). Animals that are considered more depressive tend to give up sooner and more completely when faced with a high degree of adversity.
$34.32/animal
MI1071
Rotarod Test (Maze Engineer)
This task is used to assess motor function and coordination. Motor coordination or fatigue can be evaluated by measuring the time the stays on a rotating rod before falling.
$34.32/animal
MI1072
Grip Strength Test (Maze Engineer)
This task is used to motor function and deficit. The animal's paws are placed on a wire grid, which the animal will naturally hold on to while its tail is gently pulled backwards. The maximum strength of the grip prior to grip release is recorded.
$5.39/visit
MI1073
Laboratory Assistance and Training
Laboratory Assistance and Training
$126.86/hr; surgical training + material $189.19/hr; $1,210.84/day
MI1074
Animal Housing Per diem
1) Ventilated: $1.08/mouse cage/day, $1.73/rat cage/day; 2) Non-ventilated: $1.28/mouse cage/day, $2.55/rat cage/day.
MI1075
Onsite Breeding Care
NULL
$1.97/mouse cage/day; $2.67/rat cage/day
MI1076
Technician Time
NULL
$126.86/hour
MI1077
Customized Data Analysis
NULL
$176.58/hour
MI1078
Germ Free Mice - C57BL/6
Germ Free mice are free of all exongenous micro-organisms. They can be used in the Michigan facility or shipped to other facilities in germ-free shipping containers. Unit cost is per mouse and does not include shipping.
$94.55/per mouse < 8 weeks; $128.00/per mouse > 8 weeks UofM fee (please inquire for external pricing)
MI1079
Germ Free Mice - Swiss Webster
Germ Free mice are free of all exongenous micro-organisms. They can be used in the Michigan facility or shipped to other facilities in germ-free shipping containers. Unit cost is per mouse and does not include shipping.
$81.20/per mouse < 8 weeks; $89.20/per mouse > 8 weeks UofM fee (please inquire for external pricing)
MI1080
Housing Germ Free Mice - Per Dium
The cost per day to house germ free mice.
$5.59
MI1081
Germ Free Mice - BALB/c
Germ Free mice are free of all exongenous micro-organisms. They can be used in the Michigan facility or shipped to other facilities in germ-free shipping containers. Unit cost is per mouse and does not include shipping.
$94.55/per mouse < 8 weeks; $128.00/per mouse > 8 weeks UofM fee (please inquire for external pricing)
MI1082
Germ Free Mice - IL-10 Knockout
Germ Free mice are free of all exongenous micro-organisms. They can be used in the Michigan facility or shipped to other facilities in germ-free shipping containers. Unit cost is per mouse and does not include shipping.
$94.55/per mouse < 8 weeks; $128.00/per mouse > 8 weeks UofM fee (please inquire for external pricing)
MI1083
Germ Free Mice - Rag1 Knockout
Germ Free mice are free of all exongenous micro-organisms. They can be used in the Michigan facility or shipped to other facilities in germ-free shipping containers. Unit cost is per mouse and does not include shipping.
$94.55/per mouse < 8 weeks; $128.00/per mouse > 8 weeks UofM fee (please inquire for external pricing)
MI1084
Germ Free Mice - Shipping
Germ Free Shipper is $800.00 plus the cost of shipping the animals using World Courier.
Please Inquire
MI1085
Germ Free - Technician Time
Technician time per hour for services including treatments, inoculations, fecal collections, body weight determination, blood and urine collection, etc.
$38.52/hr UofM fee (please inquire for external pricing)
MI1086
Germ Free - Per Diem
The cost per day to house germ free mice.
$6.15/ per care day UofM fee (please inquire for external pricing)
MI1092
Germ Free Quarantine per diem
All rodents shipped to the U-M from non-approved vendors and/or other institutions must be quarantined for approximately three weeks to avoid the possible introduction of infectious agents to the University’s rodent colonies. Animals in quarantine are tested for viral, bacterial, and parasitic agents. If animals test positive for rodent viral, bacterial, or parasitic infections while in quarantine, they must be treated for the infection and, if appropriate, re-derived by embryo transfer via the Transgenic Animal Model Core or used in non-survival experiments. The same is true for rodents that are known to be positive at the time of shipment to U-M. Accordingly, ULAM Veterinary Staff strongly discourages investigators from acquiring research animals with known rodent viral, bacterial, or parasitic infections. Investigators are responsible for paying all quarantine-associated costs, including technician time, laboratory testing, and per-diems. Once the animals are released from quarantine, ULAM will transfer them to the appropriate animal housing room. Study teams who wish to have animals shipped to the University of Michigan from other institutions or non-commercial (approved) vendors should contact the ULAM Rodent Health Surveillance Team at ulam-rhst@umich.edu(link sends e-mail) or (734) 936-1699 at least two weeks in advance of desired delivery date.
Please Inquire
MI1150
Body Temperature (subq, BAT, or IP) (requiring implantation of BMDS transponder)
The transponder chip (IPTT-300) will be surgically implanted in sub-cu or IP under light isoflurane anesthesia. Body temperature will be recorded using DAS7007s scanner without touching animals, Bio Medic Data Systems)
$1.79/animal
MI1151
Fear Conditioning Box
This task is used to understand the neurobiological mechanisms of fear learning and memory in rodents.
$45.45/mouse; $50.21/rat
MI1152
$191.36/procedure; $124 for the reverse surgery
Parabiosis (a surgical union of two rats)
Parabiosis (a surgical union of two rats)
MI1153
Subcutaneous implantation of temperature chips
Subcutaneous implantation of temperature chips
$13.73/animal
MI1154
IP implantation of temperature chips
IP implantation of temperature chips
$13.73/animal
MI1155
Mouse model of myocardial infarction (MI) or ischemia reperfusion injury (I/R)
A mouse model of myocardial infarction (MI) or ischemia reperfusion injury (I/R)
$348.57/mouse (recovery); $254.38/mouse (non-recovery)
MI1156
Myocardial infarction (MI) or ischemia reperfusion injury (I/R) for rat model
Myocardial infarction (MI) or ischemia reperfusion injury (I/R) for rat model
$428.39/rat (recovery); $333.93/rat (non-recovery)
MI1157
Temperature chamber housing
Animal housed in temperature controlled chamber (6~30 degrees)
$1.87/mouse cage/day; $2.66/rat cage/day
MI1158
Freezer Sample Box
Sample box used for storage at -80C
$1.93 each
MI1159
Liquid N2
A tank of liquid N2 for flash frozen tissue samples
$56.18 per tank
MI1161
Non-shivering thermogenesis with anesthesia
Non-shivering thermogenesis
$66.67/mouse
MI1162
Bomb calorimetry (Parr 6200, 1108 oxygen bomb) for measuring calorie content in food, feces, or tissue.
Samples will be collected, dried, powdered, and mixed with unbleached wheat flour or benzuic acid at 1:9 by weight to form a 1.00g pellet. Each sample will be measured in duplicate.
$60.55/animal
MI1163
Body Temperature (subq, BAT, or IP) (requiring implantation of UID transponder)
The transponder chip (UID) will be surgically implanted in sub-cu or IP under light isoflurane anesthesia. Body temperature will be continuously recorded using computer software, (Unified Information Devices)
$4.48/animal
MI1164
Germ Free 5-pack fecal kit
$24.80
MI1165
Germ Free 10-pack fecal kit
$27.00
MI1166
Germ Free 5-pack serum submandibular collection kit
$26.38
MI1167
Germ Free 10-pack serum submandibular collection kit
$23.71
MI1168
Germ Free 5-pack whole blood submandibular collection kit
$27.50
MI1169
Germ Free 10-pack whole blod submandibular collection kit
$33.95
MI1170
Germ Free Injection kit
$23.85
MI1171
Germ Free Gas sterilization – small
$29.70
MI1172
Germ Free Gas sterilization – medium
$25.50
MI1173
Germ Free Gas sterilization – large
$35.30
MI1174
Germ Free Sterile mouse box transport kit
$27.65
MI1175
Germ Free Oral gavage w/ feeding needle kit
$32.29
MI1176
Germ Free Port entry/exit
$30.40
V3000
Jugular Vein or Carotid Artery Catheterization
Jugular Vein or Carotid Artery Catheterization (please specify in your order).
Inquire for Pricing
V3001
Cannulation of Cerebral Ventricle
Implantation of a cerebral ventricle cannula allows investigators to evaluate physiological responses following central administration of various compounds. Anesthetized mice are placed in a digital stereotaxic apparatus (0.001 mm accuracy, Cartesian Instruments) specifically designed for mice. The dorsal scalp will be shaved, wiped with a betadine solution, and then a small midline incision over the dorsal surface is made to allow access to the cranium. After the affixed centering scope is used to "zero" lambda and bregma landmarks, a single guide cannula (2.5 mm length, 26-gauge, Plastics One) is positioned 1.0 mm above the lateral ventricle (coordinates: 0.6 mm posterior to bregma, 1.5 mm lateral to midline, 1.4 mm below the surface of the skull) and fixed to the skull using two stainless steel screws and dental cement. The incision in the scalp is then closed with surgical thread. Animals are removed to a post-surgical warming bed, and then individually housed for several hours until fully awake. Animals will be allowed to recover from surgery for a minimum of 7 days prior to testing, during which time a 30-gauge dummy cannula is left inside the guide cannula to prevent blockage.
Inquire for Pricing
V3002
Jugular vein and carotid artery catheterization
Arterial catheterization allows investigators to sample arterial blood as required for adequate glucose clamping (Niswender et al. J. Biol. Chem, 1997, Halseth et al. Am. J. Physiol. 1999) or other infusion/sampling purposes (Rottman et al. Am. J. Physiol. 1999) Catheterization of the right jugular vein allows the infusion of hormones, substrates, and tracers into the systemic circulation. Catheterization of the left carotid artery allows for arterial blood sampling without handling the mouse. The total duration of the operation is about 40 min. Experiments are typically scheduled 5-7 days after surgery.
Inquire for Pricing
V3003
Glucose Tolerance Test (Oral, i.p., Intravenous, or gastric catheter)
Oral glucose tolerance tests are performed on conscious mice and can be performed with catheters chronically implanted directly in the stomach and the carotid artery. Intravenous glucose tolerance tests are performed on conscious mice with catheters chronically implanted in the jugular vein and carotid artery. Glucose will be given at 1g/kg or 2g/kg. These doses lead to peak blood glucose levels of 250 mg/dl to 400 mg/dl in wild type C57/bl/6 mice.
Inquire for Pricing
V3004
Glucose turnover
A primed continuous infusion of [3-3H]glucose or 2D2-glucose is used to assess the rates of glucose appearance (Ra) and disappearance (Rd). Tracer is infused to allow a steady state to be reached then blood samples are taken to assess arterial glucose specific activity. Application of this technique is described by Niswender et al. J. Biol. Chem. 1997, and She et al. Mol. Cell. Biol. 2000.
Inquire for Pricing
V3005
Hyperinsulinemic clamp
The hyperinsulinemic clamp is used to measure insulin action in vivo. Hyperinsulinemic clamps are performed on conscious mice with catheters chronically implanted in the jugular vein and carotid artery. A continuous infusion of insulin is given. Glucose levels are monitored in arterial samples every 10 min using a glucose analyzer that allows the analysis of glucose with only 1 µl of blood. Glucose is infused in the jugular vein catheter at rates necessary to achieve the desired glucose level, based on feedback from arterial glucose measurements. These methods allow assessment of the responsiveness of the body to insulin. Blood from a donor animal is infused to maintain blood volume. By combining this technique with the tracer method one can also examine the impact of insulin on suppression of endogenous glucose production. (1, 2) 1. Ayala JE, Bracy DP, Malabanan C, James FD, Ansari T, Fueger PT, et al. Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice. J Vis Exp. 2011:e3188. 2. Halseth AE, Bracy DP, Wasserman DH. Overexpression of hexokinase II increases insulin and exercise-stimulated muscle glucose uptake in vivo. Am J Physiol. 1999 1/1999;276(1 Pt 1):E70-E7.
Inquire for Pricing
V3006
Hyperglycemic clamp
The responsiveness of the pancreas to glucose is assessed using the hyperglycemic clamp. Hyperglycemic clamps are performed on conscious mice with catheters chronically implanted in the jugular vein and carotid artery. Our clamps include 11 arterial insulin measurements and 4 arterial c-peptide measures. A defined hyperglycemic stimulus is created using a primed variable glucose infusion to raise the glucose level to twice basal for 120 min. An established priming algorithm is used to elevate glucose quickly.
Inquire for Pricing
V3008
Glycogen synthesis
Using radioactive or stable isotope labelled glucose, the incorporation of the carbon of glucose into glycogen can be measured.
Inquire for Pricing
V3009
Amino acid kinetics
The turnover of phenyalanine (3H ring 2,3,4,5,6 phenylalanine) , glutamine (U-14C-glutamine) and leucine (1-14C-leucine) is assessed by a primed continuous infusion of their respective isotopes for 2 hours (0.2-0.4 µCi/min). Blood samples (20 µl) are taken after a steady state is reached to assess plasma amino acid specific activity. Blood samples are mixed with an equal volume of 6% sulfosalicylic acid. Incorporation of tracer in tissue protein is used to assess tissue specific protein synthesis.
Inquire for Pricing
V3010
Tissue specific glucose uptake
Tissue specific glucose uptake is assessed by measuring the tissue specific uptake of [2- 3H]-deoxyglucose([2-3H]DG). [2-3H]DG is infused for 40 minutes or injected as bolus. Arterial plasma samples are taken to determine the time course of [2-3H]DG during the 40 min period. [2-3H]DG is transported into cells and phosphorylated to yield [2-3H]DG-6-phosphate which is trapped in muscle. After 25-40 min mice are anesthetized with an intravenous infusion of pentobarbital and tissues of interest are rapidly removed and frozen in liquid nitrogen. This method has been applied during insulin- and exercise-stimulated conditions (Halseth et al. Am. J. Physiol. 1999).
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V3012
Indirect calorimetry / energy expenditure in the Promethion
Whole body VO2 and VCO2 is measured continuously in conscious mice using a Promethion system (Sable Systems Int). The system is sensitive enough to measure small changes in VO2, VCO2 and RQ. They can be used to measure resting or exercising (running wheels or treadmill) gas exchange and energy expenditure. The Promethion is very advanced, allowing for measurement and control of food or water intake. In the Promethion the animals are housed in regular home cages with normal bedding. Activity is measured using beam breaks and converted to pedestrian locomotion. Food and water intake are very accurately quantified. The system integrates all of these data to monitor behaviors and patterns. Body weight and composition is included and measured before and after the metabolic cages. All mice remain in the Promethion cages for 5 days with continuous data collection, allowing for acclimation while also providing extensive data. Full data is reported to the investigator in excel format.
Inquire for Pricing
V3013
Exercise capacity/Exercise Stress Test (metabolic response to exercise)
Exercise is an integrated measure of fitness. Abnormal exercise capacity and decreased activity are a hallmark of most severe cardiovascular and metabolic diseases, and changes in exercise capacity are sensitive and early markers of cardiac and metabolic dysfunction. Thus abnormalities can be revealed with exercise that may not otherwise be manifested. Gas exchange techniques can be used during treadmill exercise in the mouse to describe the metabolic cost of exercise. Substrate fluxes and metabolism can be assessed isotopically during exercise in chronically catheterized mice (Halseth et al. Am. J. Physiol. 1999). Treadmill exercise can be used to quantify the capacity of a mouse for either endurance or high intensity exercise. Peak exercise capacity and VO2 max will be measured using a closed gas exchange treadmill. Acclimated mice will exercise will at 10 m/min, 0° grade, increased to 14 m/min, 3 minutes later and then increased by 4 m/min a every 3 min thereafter up to 46 m/min or until mouse is exhausted. Exhaustion is defined as the mouse sitting on the shock pad and unable to get off. Time to exhaustion and speed at exhaustion is recorded.
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V3014
Spontaneous exercise activity
Spontaneous exercise activity is measured using a recording wheel placed in the cage during a 48 h period. The light dark cycle will be stringently controlled to minimize diurnal variations and training effects will be minimized by placing an identical wheel in the cage for the 24 hrs preceding the test measurement. Variables recorded include total distance traveled, peak speed and exercise duration.
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V3015
Food Consumption
Food consumption is assessed using an automated feeding apparatus that continually measure feeding behavior in an unobtrusive manner by allowing animals free access to food cups that are mounted on balances. The apparatus currently is capable of measuring and time-stamping individual weights from 16 balances simultaneously every 30 seconds and downloading the data directly to a computer for subsequent analysis. Therefore, cumulative food consumed and the time at which feeding bouts occur are continuously monitored. All feeding studies are done after the animal has acclimatized to the facility for at least 24 hours.
Inquire for Pricing
V3016
Exploratory locomotor activity
Exploratory locomotor activity
Inquire for Pricing
V3017
Assess real time imaging of cellular metabolic events
Assess real time imaging of cellular metabolic events will be done.
Inquire for Pricing
V3018
In vivo optical imaging of gene expression
In vivo optical imaging of gene expression will be done.
Inquire for Pricing
V3032
Chronic arterial blood pressure measurement (telemetry)
Because of difficulties in making physiological measurements in anesthetized mice, a commercially-available system for recording mean arterial blood pressure, heart rate, systolic, diastolic, and mean pressure are used. The primary probe is the PA-C10 which is completely implantable, reducing animal stress, and ensuring the most reliable data. Probes to measure ECG and blood glucose are also available.upon request. This service includes probe implantation, probe removal, data acquisition, and a partial probe refurbishment fee.
Inquire for Pricing
V3033
Acute arterial blood pressure (via arterial catheter)
Blood pressure (BP) represents an integrated measure of overall cardiovascular function, and is affected by stroke volume, heart rate, inotropic state, and vascular tone. Abnormalities of BP regulation (primarily hypertension, but also hypotension) are associated with major cardiovascular morbidity and mortality, and are epidemiologically associated with diabetes and obesity.
Three complementary technologies are available for the measurement of BP: 1) non-invasive tail-cuff plethysmography, 2) direct arterial measurement by intracarotid catheterization, and 3) telemetry via implanted catheter.
(1) Tail-cuff Plethysmography. Plethysmography (tail-cuff) is performed using a tail-cuff BP apparatus (BP-2000, Visitech Systems, Inc.). This technology is non-invasive and there is good concordance with the direct BP measurements described below.
(2) Carotid Catheterization. Direct arterial measurements are obtained via a chronically placed catheter in the carotid artery. The catheter is connected to a TXD-310 transducer and BP measured using a Digi-Med BPA 400 (Micromed). Experiments using this approach are typically coordinated with metabolic measurements.
A dual catheter approach (arterial and venous) allows for BP measurements in response to specific pharmacological infusions in the awake or anesthetized state; and enables evaluation of both peripheral and central mechanisms of BP regulation.
(3) Telemetry. Telemetered direct BP measurement is performed using an implanted micro-miniature device (PA-C10, DSI) implanted subcutaneously with the catheter typically placed in the right carotid artery. The mouse is housed individually in a cage placed over the receiver platform and BP data digitally recorded via the DATAquest A.R.T. system (DSI). Advantages of this approach include the ability to continuously record BP over a period of weeks, to assess the diurnal range in BP, and stress artifact induced by animal handling is avoided.
Inquire for Pricing
V3035
Osmotic Pump Implant
Osmotic pump is implanted in mouse.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3050
Insulin
30-100 ul plasma for duplicate analysis is typical, but the required sample volume depends on the hormone concentration and dilution factor. The assay range is 0.01-0.5 ng/ml (sensitive assay) and 0.1-10 ng/ml (regular assay) with 300 ul (so with 30 ul the detection range is 0.1-5 ng/ml for the sensitive assay and 1-100 for the regular assay). 5-day double antibody assay. Interspecies and human-specific assays are available.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3052
Corticosterone
15 µl plasma for duplicate analysis. 1-day double antibody assay. Detection range: 25-1000 ng/ml.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3054
Leptin (Luminex assay)
Available as a Luminex assay. The first analyte within a panel is $39.93 and each additional analyte within the same panel is $6.60, per sample in duplicate (e.g., to measure three analytes from panel 1= $39.93+$6.60+$6.60 and two analytes from panel 2 = $39.93+$6.60)
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3055
C-peptide (Luminex assay)
Available as a Luminex assay. Needs EDTA/Trasylol added at the time of collection. The first analyte within a panel is $39.93 and each additional analyte within the same panel is $6.60, per sample in duplicate (e.g., to measure three analytes from panel 1= $39.93+$6.60+$6.60 and two analytes from panel 2 = $39.93+$6.60)
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3056
Growth Hormone (GH)
Available as Luminex assay.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3065
Adiponectin
Adiponectin may be analyzed in a double-antibody RIA format. It requires an overnight incubation at room temperature. Sample volume < 2 µL serum or plasma, or < 100 µL tissue culture media. A 1:400 x dilution is required. The lower limit of detection is 1.56 pg/mL. Can also be run as single-plexed assays using luminex instrumentation. A dilution step is required for the adiponectin requiring a minimum of 5 ul of sample. The resistin does not require a dilution and may be multiplexed with other adipokines with a required sample volume of 25 ul for duplicate analysis.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3070
Plasma lipids
Enzymatic Free Fatty Acid Test Price - $15.60
Total plasma cholesterol and triglyceride are measured by standard enzymatic assays. HDL cholesterol is measured with the enzymatic method after precipitation of VLDL and LDL using dextran sulfate and Mg++. Using these data LDL cholesterol can be calculated using the Friedewald equation, if triglyceride levels are below 400 mg/dl. Investigators may request a total plasma lipid profile or specific plasma lipid measurements.
Free fatty acids are extracted from plasma using heptane/isopropanol. The heptane layer containing FFA is removed, plated on silica gel plates and developed in petroleum ether, ethyl ether, and acetic acid. The FFA band is scraped from the plate and FFA are eluted with heptane /isopropanol. The solvent is removed, and the FFAs are methylated. Methylated fatty acids are analyzed by gas chromatography. Depending on the assay a variety of chromatograph conditions and columns are utilized. A computer identifies each fatty acid peak and can provide data in a number of different ways including quantitation of mass of fatty acid, percent distribution of fatty acids present, quantitation of total lipid in the sample.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3071
Lipid extraction, separation, quantification
Total plasma cholesterol and triglyceride are measured by standard enzymatic assays. HDL cholesterol is measured with the enzymatic method after precipitation of VLDL and LDL using dextran sulfate and Mg++. From these data LDL cholesterol is calculated using the Friedewald equation, as long as triglyceride levels are below 400 mg/dl. Investigators may request a total plasma lipid profile or specific plasma lipid measurements. Plasma FFAs are analyzed with a commercially available enzymatic kit from Wako Life Sciences. Free fatty acids are extracted from plasma using heptane/isopropanol and separated from other lipid classes by thin layer chromatography. The FFAs are methylated and analyzed by gas chromatography. Data: (1) total FFAs present (mg/ml plasma); (2) percent distribution of individual fatty acids in the FFA fraction. Tissue/Cell Lipid Analyses (Phospholipid, Diglycerides, Free Fatty Acids, Triglycerides, and Cholesterol Esters)
Lipids are extracted by the Folch-Lees method. Internal standards are added, and the lipid classes are separated by thin layer chromatography. The appropriate band(s) are scraped from the TLC plate, and the fatty acids from the lipid ester classes are methylated and analyzed by gas chromatography. Results: (1) total amount of lipid class present; (2) fatty acid profiles of the lipid classes presented as percent of total fatty acids. Total unesterified cholesterol is analyzed in the lipid extract by gas chromatography after addition of appropriate internal standard. An aliquot of the lipid extract is dried and saponified at 80 C in 1 N KOH in 90% methanol for 1 hour. The non-saponifiable sterol is extracted into hexane, concentrated under nitrogen, solubilized in carbon disulfide and analyzed by gas chromatography.
Lipoprotein fractions are separated from 100 microliters of plasma (or serum) by gel filtration column chromatography. Approximately 40 fractions (0.5 ml) are collected and the amount of triglyceride and cholesterol in each fraction is determined using microtiter plate, enzyme-based assays. Profiles of triglyceride and cholesterol are constructed. Calibration of the column with purified lipoprotein fractions permits quantitation of each lipid in various lipoprotein classes.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3072
Fatty acid profiles of lipid esters by gas liquid chromatography
Total lipids are extracted and lipid classes separated by TLC as described above. Lipid ester spots are scraped from the plates and methylated. Fatty acids of lipid esters can be methylated without removal of the lipid from the silica gel. However, in some applications, we have found it advantageous to elute the lipid from the silica gel prior to methylation. The fatty acid profile of the lipid class is also determined. By this method total lipid mass and fatty acid profile for each lipid is determined.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3073
Quantitation of individual phospholipid classes
Individual phospholipid classes are isolated by one dimensional TLC. A total lipid extract is applied to high performance TLC plates. To quantitate the individual classes, the spots are scraped from the plate, eluted and phosphorus is determined. To determine the fatty acid composition of the individual phospholipid classes, the spots are scraped from the plates and fatty acids methylated.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3074
Short chain fatty acid analysis by gas liquid chromatography
Plasma short chain fatty acids are analyzed by the following procedure: to 200 ul of EDTA plasma in a 1.5 ml Eppendorf microfuge tube is added 20 µl of internal standard and 1 ml of absolute ethanol. The sample is mixed thoroughly, centrifuged, and the supernatant is recovered. The sample is evaporated using a Speed Vac and dissolved in 15 µl water, and prior to injection 5 µl of orthophosphoric acid (25%) is added. The short chain fatty acids are separated on a 6' x 2 mm glass column packed with SP-1200/1%H3PO4 on 80/100 Chromosorb W AW.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3075
Fast Protein Liquid Chromatographic (FPLC) Lipoproteins Separations
Lipoprotein fractions are isolated using columns arranged in tandem to achieve complete resolution of the major lipoprotein classes from 1-2 ml of plasma. The columns are equilibrated in 50 mM phosphate-buffered saline and calibrated using lipoprotein fractions isolated by ultracentrifugation. Fractions (0.5 ml) are collected and the appropriate tubes containing the desired lipoprotein fraction(s) combined. The position of the major lipoprotein classes are determined by cholesterol (or triglyceride) assay on the column fractions using a microtiter plate enzyme-based assay. As an alternative method lipoproteins can be isolated by fast protein liquid chromatography.
This includes analysis of the composition of the fraction (protein and lipid) as well as morphologic analysis (sizing) by negative stain electron microscopy. For compositional analysis the lipoprotein fractions protein is analyzed using the BCA method with a modification to eliminate lipid interference. The samples are then lyophilized and delipidated using ethanol and ether. Lipid components are separated by TLC and analyzed by GLC and/or colorimetric assays.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V3080
Gross examinations and necropsy
The standard necropsy procedure for diabetic mice includes an examination of the pancreas, heart, liver, eyes, peripheral nerves, peripheral vasculature, fat, and kidneys. Complete, intermediate, or limited necropsies will be performed with or without the aid of a dissecting microscope. Gross pathologic findings will be described, documented by digital photography and organs will be weighed.
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V3090
Amino Acids - Full Profile (HPLC)
50 ul plasma or supernatant from tissue prep (not urine). Detection range: >1 umol/l.
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V3091
Amino Acid - gluconeogenic profile
50 ul plasma or supernatant from tissue prep (not urine). Detection range: >1 umol/l.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V4007
Surgical Training
Surgical training for a variety of surgery techniques (catheterization, telemetry, bariatric surgery, implants)
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V4010
Ghrelin - Active (RIA)
30-60 ul plasma for duplicate analysis is typical, but the required sample volume depends on the hormone concentration and dilution factor. The assay range is 5-100 pg/ml with 300 ul (so with 30 ul the detection range is 50-1000 pg/ml). 5-day double antibody assay. Needs Pefabloc SC (AEBSF) added at the time of collection.
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V4011
Ghrelin - Total (RIA)
30-60 ul plasma for duplicate analysis is typical, but the required sample volume depends on the hormone concentration and dilution factor. The assay range is 100-1000 pg/ml with 300 ul (so with 30 ul the detection range is 1000-10,000 pg/ml). 5-day double antibody assay.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V4012
Glucose (Enzymatic)
Glucose is measured by a glucose oxidation method using the Analox GM9 Glucose Analyzer. 40 ul of plasma for duplicate analysis. Detection range: 20-900 mg/dl.
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V4017
Luminex Assays
Mouse Single Plex Adiponectin Panel
Mouse Cytokine/Chemokine Panel 1
Mouse/Rat IGF-1 Panel
Mouse Th17 Panel
Mouse High Sensitivity T Cell
Mouse Metabolic Hormone Panel
These are most frequently used assays. For more information, go to https://www.vumc.org/hormone/assays
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V4019
Cortisol - Plasma
Cortisol - Plasma
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V4021
Creatinine
30 ul plasma minimum
Inquire for Pricing. Analytical services are provided as part of in vivo projects.
V4024
Bariatric Surgery (Roux-en-Y, VSG, biliopancreatic diversion, sham controls)
Bariatric surgery (Roux-en-Y gastric bypass, vertical sleeve gastrectomy, biopancreatic diversion, and appropriate sham controls) . The modified gastric bypass in the mouse: In this procedure the stomach is bypassed and the food flows through the bypass arm directly into the jejunum.
The biliopancreatic diversion procedure in the mouse: This procedure is historically thought to be a malabsorptive procedure and has several variations in the human. In brief, the biliary and pancreatic secretions are physically separated from gastrointestinal chyme flow until a point near the terminal small bowel. In theory this leads to significant malabsorption, though when this procedure is done clinically many times a gastric restriction component is also added.
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V4026
Body Composition Analysis by NMR
Body composition analysis by NMR analysis (Bruker Minispec). This measurement does not require anesthesia of the mouse. Body composition will provide lean mass, fat mass, total body weight, adiposity (%) and % lean mass for each mouse.
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V4034
Exercise training (5x/wk)
Mice undergo a training regimen up to 5 days per week, 1 hour daily, on a treadmill. Typical speeds are ~ 40% VO2max or lower.
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V4035
Voluntary exercise (running wheels)
Running wheels can be placed in regular housing cages
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V4036
Core body temperature monitoring
A temperature-recording pill (anipill) is surgically implanted in the ip cavity. Data is recorded every 5-60min for days or weeks.
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V4037
Thermoneutral housing or cold stress
Thermoneutral housing or cold stress
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V4038
Bariatric Surgery (Roux-en-Y Gastric Bypass (RYGB & Sham))
The RYGB procedure involves creating a small gastric pouch and connecting it directly to the jejunum, bypassing a significant portion of the stomach and the duodenum.
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V4039
Glucose Clamping the Conscious Mouse: A Laboratory Course
Five-day hands-on course focusing on mouse jugular vein and carotid artery catheterization, clamp techniques, and tracer methods. For more information, email mmpc@vanderbilt.edu
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V4040
Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis
Week-long course on the theory and practice of stable and radioactive isotopic tracers for the study of metabolism in human, animal and cell models. For more information, visit https://www.isotopetracercourse.com/
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V4041
Mouse Kidney Injury Workshop
Five-day, hands-on, supervised training workshop focused on kidney injury models, surgical techniques, tissue and body fluid collections, and renal function tests in mice. Course offered in collaboration with the VUMC O'Brien Kidney Center. For more information, visit https://medsites.vumc.org/vanderbiltobrienkidneycenter/vokc-workshops
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V4042
Study Consultation
Meet with Vanderbilt MMPC faculty to discuss your project, potential future studies, or educational opportunities
$0.00
V4043
Frequently sampled IV GTT (FSIVGTT)
Glucose is delivered as an intravenous bolus and arterial blood samples are rapidly collected (every 1-5min). Measures of insulin action can be calculated using the Bergman Minimal Model adapted to the mouse. Requires catheters.
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V4044
Islet transplant under the kidney capsule
Islet transplant under the kidney capsule
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V4045
Islet transplant in the anterior chamber of the eye
Islet transplant in the anterior chamber of the eye
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V4046
Islet isolation (from mice)
Pancreas is perfused and digested, islets are isolated and picked for subsequent analyses.
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V4047
Islet hormone secretion (perifusions)
Islet perifusions are carried out using the Peri-Lite system (4 chambers).
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V4048
Assessment of islet calcium handling
Changes in beta cell intracellular calcium concentration are critical to insulin release. This assay relies on fluorescent microscopy to visualize and measure changes in intracellular calcium concentration within islet cells.
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V4049
In vivo insulin pulsatility
In a clamped hyperglycemic state, plasma insulin is measured every minute for 15min in the conscious free-roaming mouse. Requires catheters.
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V4050
Hypoglycemic clamp
The counter-regulatory response to hypoglycemia is assessed using the hypoglycemic clamp. Hypoglycemic clamps are performed on conscious mice with catheters chronically implanted in the jugular vein and carotid artery. Our clamps include glucagon, catecholamines and corticosterone measures over time. A defined hypoglycemic stimulus is created using an insulin infusion and a variable glucose infusion to lower the glucose level to ~50-70 mg/dL for 120 min. The rate of fall is controlled across animals.
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V4051
Adipose tissue transplantation
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V4052
Gonadectomy (ovariectomy, orchiectomy)
Gonadectomy (ovariectomy, orchiectomy)
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V4053
Oral catheter implantation
For stress-free delivery of substances to the tongue of the mouse.
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V4054
Tracer infusions in the conscious unrestrained mouse
A variety of radioactive or stable isotope tracers can be infused in the conscious stress-free mouse through a jugular vein catheter. Please inquire to discuss your study design.
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V4055
i-STAT arterial blood gases
Measures in a single arterial sample pCO2, pO2, Na, K, ionized Ca, Glucose, hematocrit, hemoglobin, pH, TCO2, HCO3, sO2.
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V4056
Plasma lactate
Colorimetric or fluorometric assay
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V4057
Blood ketones
Blood Ketones are measured on a fresh drop of blood using a ketone meter
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V4058
Glomerular filtration rate
Transdermal method in conscious mice. Involves attaching a miniaturized fluorescence detector to the mouse's skin and monitoring the excretion kinetics of FITC-sinistrin (inulin analog) after iv injection. This allows for non-invasive, repetitive measurements of GFR in conscious mice.
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Y4001
Hyperinsulinemic-euglycemic clamp experiments
The surgery is performed at 4-5 days prior to the hyperinsulinemic-euglycemic clamp to establish a chronic catheter for intravenous infusion of substances (e.g., glucose, insulin) during the clamp. For this, a mouse is anesthetized with an intraperitoneal injection of ketamine and xylazine, and a catheter is inserted in the right jugular vein. On the day of clamp experiment, an overnight-fasted mouse is placed in an over-sized restrainer (i.e., rat-sized) for the experiment to be conducted in awake and minimally-stressed state. The tail is tethered using a tape for 2 hours prior to the start of experiment for acclimatization. A 3-way connector is attached to the jugular vein catheter for intravenous infusion, and the blood samples are obtained from the tail vessels requiring a small tail cut. A 2-hour hyperinsulinemic-euglycemic clamp is conducted with a primed-continuous infusion of human insulin at a rate of 15 pmol/kg/min to raise plasma insulin within a physiological range (~300 pM). Blood samples (20 ml) are collected at 10-20 min intervals for the immediate measurement of plasma glucose concentration, and 20% glucose is infused at variable rates to maintain glucose at basal concentrations (~6 mM). Insulin-stimulated whole body glucose metabolism is assessed with a continuous infusion of [3-3H]glucose (0.1 mCi/min) throughout the clamps. Basal rates of whole body glucose turnover are assessed using a primed-continuous infusion of [3-3H]glucose for 2 hours prior to the start of clamp. All infusions are performed using the microdialysis pumps, and all procedures are approved by Yale University Animal Care and Use Committee. To estimate insulin-stimulated glucose uptake in individual tissues, 2-deoxy-D-[1-14C]glucose (2-[14C]DG) is administered as a bolus (10 mCi) at 75 min after the start of clamp. Blood samples (20 ml) are taken at -5, 80, 85, 90, 100, 110, and 120 min of clamp for the measurement of plasma [3H]glucose, 3H2O, and/or 2-[14C]DG concentrations. Additional blood samples (20 ml) are collected before and at the end of clamp for the measurement of plasma insulin concentrations. At the end of clamp, mouse is anesthetized with sodium pentobarbital injection, and tissues are taken and stored for biochemical/molecular analysis. The clamp experiment measures tissue-specific insulin action and glucose metabolism and includes the following measurements: 1) basal and insulin-stimulated hepatic glucose production, 2) insulin-stimulated whole body glucose uptake, glycolysis, and glycogen plus lipid synthesis, and 3)insulin-stimulated glucose uptake, glycolysis, and glycogen synthesis in individual tissues (e.g., skeletal muscle, adipose tissue, heart). Additionally, biochemical/molecular assays may be performed to assess tissue-specific insulin signaling activities (e.g., insulin-stimulated tyrosine phosphorylation of IRS, PI 3-kinase activity) and tissue-specific triglyceride contents. Further details of the clamp experiment can be found in the following references: Diabetes 53:1060 (2004), J. Clin. Invest. 114:823 (2004).
$600.00
Y4002
14C-2-Deoxylglucose Uptake in Muscle and Adipose Tissue
To estimate insulin-stimulated glucose uptake in individual tissues, 2-deoxy-D-[1-14C]glucose (2-[14C]DG) is administered as a bolus (10 mCi) at 75 min after the start of clamp
$462.00
Y4003
Hyperglycemic clamp
The procedure involves chronic cannulation of the jugular vein which is necessary for The surgery is performed at 4-5 days prior to the hyperglycemic clamp to establish a chronic catheter for intravenous infusion of substances (e.g., glucose, insulin) during the clamp. For this, a mouse is anesthetized with an intraperitoneal injection of ketamine and xylazine, and a catheter is inserted in the right jugular vein. On the day of clamp experiment, an overnight-fasted mouse is placed in an over-sized restrainer (i.e., rat-sized) for the experiment to be conducted in awake and minimally-stressed state. The tail is tethered using a tape for 2 hours prior to the start of experiment for acclimatization. A 3-way connector is attached to the jugular vein catheter for intravenous infusion, and the blood samples are obtained from the tail vessels requiring a small tail cut. A 2-hour hyperglycemic clamp is conducted with a variable infusion of 20% glucose to raise and maintain plasma glucose concentrations at ~16 mM. Blood samples (20 ml) are collected at 10-20 min intervals for the immediate measurement of plasma glucose concentrations using Beckman Glucose Analyzer. The area under curve of plasma glucose and insulin profiles is assessed to determine glucose-induced insulin secretion in vivo (i.e., pancreatic ß-cell function).
$430.00
Y4004
Basal multiple tracer infusion studies
We are able to perform infusions to measure the turnover of a variety of substrates in vivo. Please inquire about tests we have performed or to design a custom experiment.
$380.00
Y4005
Basal Tracer Infusion
Infusion Studies used to measure turnover of various substrates (stable and/or radioacticve) and require continuous infusion of various agents in awake mice.
$200.00
Y4006
Metabolic Cages
Mice are housed in the Columbus Labs Animal Monitoring System (CLAMS) to determine energy expenditure by indirect calorimetry, physical activity, feeding behavior and drinking behavior. Respiratory exchange ratio and body composition by proton NMR are also included in the analysis.
$100.00
Y4007
Treadmill Cages (VO2 Max/Fatigue tests)
Mice are housed in treadmills in the Columbus Labs Animal Monitoring System (CLAMS) to determine energy expenditure by indirect calorimetry during exercise. Respiratory exchange ratio and body composition by proton NMR are also included in the analysis.
$57.00
Y4008
Intraperitoneal Glucose Tolerance Test
Mice are fasted overnight prior to collection of a basal sample of plasma for determination of glucose and insulin levels. Mice are then given a 1 g/kg body weight injection of glucose. Plasma is taken at set time points for 120 min to determine plasma glucose and insulin levels.
$113.00
Y4009
Intraperitoneal InsulinTolerance Test
Mice are fasted overnight prior to collection of a basal sample of plasma for determination of glucose and insulin levels. Mice are then given a 0.5 U/kg body weight injection of insulin. Plasma is taken at set time points for 120 min to determine plasma glucose and insulin levels.
$122.00
Y4010
Oral Gavage Glucose Tolerance Test
Mice are fasted overnight prior to collection of a basal sample of plasma for determination of glucose and insulin levels. Mice are then given an oral gavage (1 g/kg body weight) of glucose. Plasma is taken at set time points for 120 min to determine plasma glucose and insulin levels.
$110.50
Y4011
Body Composition by MR (labor only)
Mice undergo body composition measurements using proton NMR. This technique measures the resonance frequency of protons in the mouse and returns a value that corresponds directly to protons in triglyceride (whole body fat mass) and a value that corresponds to protons in body water, from which a calculation of lean mass (including muscle, liver, kidney, brain, etc…) is made based on reference standards. Therefore, the numbers you receive correspond to whole-body fat (not a specific fat pad).
$9.50
Y4012
Basal Blood Draw
Mice are fasted overnight or 6 hours. Mice are then placed in an over-sized restrainer (i.e., rat-sized) for the experiment to be conducted in awake and minimally-stressed state. The tail is tethered using a tape for 2 hours prior to the start of experiment for acclimatization. Following this, the blood samples are obtained from the tail vessels requiring a small tail cut
$60.00
Y4013
Necropsy/tissue collection
Mice are first put under isoflurane anesthesia. Tissues are collected and immediately frozen in liquid nitrogen and stored at -80°C.
$10.50
Mouse Phenotyping