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Strain
B6.129S1-Dnm2tm1.1Pdc Tg(Tek-cre)12Flv

Summary Data Summary
Official Name B6.129S1-Dnm2tm1.1Pdc Tg(Tek-cre)12Flv
Common Name B6.129S1-Dnm2tm1.1Pdc Tg(Tek-cre)12F
Description Dnm2 conditional knockout targeting strategy was previously
reported (Ferguson et al., 2009). EC Dnm2-deficient mice
were generated by crossing Dnm2flox/flox females with
Dnm2flox/+; Tie2Cre males (Jackson Laboratory).

Dnm2 flox: A loxP site was inserted upstream of exon 2 and
an frt flanked neo cassette with a 3' loxP site was inserted
downstream of exon 2. Flp mediated recombination removed the
neo cassette leaving exon 2 floxed.

Tg(Tek-cre): Cre recombinase expression is driven by a mouse
endothelial-specific receptor tyrosine kinase
promoter/enhancer. Recombinase activity is detected in
endothelial cells during embryogenesis and adulthood.
Expression was also detected in hematopoietic cells.
Development Status Phenotyping ongoing
Creation Method knockout
TypeCount
Investigators 1
Genomics - Modifications 1
Genomics - Transgenes 1
Experiments 1


Investigators
NameInstitution
Monica LeeYale University


Genomic Information
GeneAllele 1Allele 2Protocol
Dnm2knockoutknockoutNot Specified
EnhancerPromoterGeneCopies
Not SpecifiedTekCRENot Specified


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