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University of California Davis
http://mmpc.ucdavis.edu

Description	Provides tests for metabolism, insulin resistance, glucose balance, energy balance, eating behavior and cardiovascular phenotyping,
Director / Email	K.C. Kent Lloyd, DVM, Ph.D. - kclloyd@ucdavis.edu
General Contact	mmpc@ucdavis.edu
Grant Number	DK092993
RRID	SCR_015357 (Cite This)

Center Cores:

	Center Core	Core Description	RRID
	Center: University of California Davis; Director: K.C. Kent Lloyd, DVM, Ph.D.
	Animal Care, Surgery, and Pathology Core	The Core provides extensive scientific support services, including importation/exportation, colony management, customized mouse models, rederivation and creation of genetically-modified mutant mice (e.g. CRISPR), and re-animation of a variety of models cryopreserved within the KOMP or MMRRC repositories. These new and extant mouse models can then be bred to produce male and female cohorts for in vivo and in vitro phenotyping, including rederivation into our Gnotobiotic Mouse Research Center for gut microbiome studies. The Core also operates a Microsurgery Suite, with two expert microsurgeons, for development of surgically-manipulated mouse models, such as bariatric surgical models, e.g. Vertical Sleeve Gastrectomy (VSG) and Roux en Y Gastric Bypass (RYGB), as well as cannulation telemetry and osmotic pump implants, in addition to providing microsurgery training services for investigators and clinicians. In addition to testing, manipulation, and sampling, investigator can have these surgically-manipulated models as well as any genetically-engineered mouse lines shipped from our approved vendor barrier vivarium directly into the recipient institution’s vivarium. The Core also provides ancillary services and procedures, including blood sampling for in vivo testing for glucose tolerance tests and insulin tolerance tests. We provide pathology analysis to complement other testing including survey necropsy with histology, clinical chemistry and hematology analysis. This testing can be customized to include specific organ histologic examination, grading and photo documentation. The Core is led by Kristin Grimsrud DVM, PhD, who oversees two trained rodent surgical technicians, one import/export coordinator, and two phenotyping technicians, and is co-led by Stephen Griffey DVM, PhD.	SCR_015356
	Endocrinology and Metabolism Core	The Endocrinology and Metabolism Core provides phenotyping services for the assessment of endocrine function and metabolic pathways in mouse models of obesity, diabetes, and related metabolic diseases including dyslipidemia and fatty liver disease (NAFLD). The Core also provides expertise, technical resources, and instrumentation necessary to characterize perturbations in endocrine systems and metabolism in murine models useful for understanding obesity, diabetes, its complications, and related metabolic disorders, including NAFLD. The Core performs assays and data interpretation for in vivo metabolic function tests, including IV, IP and oral glucose tolerance tests and insulin tolerance tests for parameters of insulin sensitivity, insulin secretion and glucose disposal. In addition, the Core offers an extensive list of quality controlled assays of metabolic substrates, endocrine hormones, and indices of renal function, assessments of insulin signaling pathways, inflammation and endoplasmic reticulum stress in metabolically important tissues such as liver, muscle, adipose, pancreas, as well as state-of-the-art metabolomic analysis and interpretation, including complex lipids, phospholipids, bile acids, and biogenic amines using small sample volumes. The Core works closely with the Animal Care Core on projects evaluating the metabolic effects of bariatric surgery, and with the Microbiome Core to coordinate microbiome and metabolomic analyses and interpretation. Continuous glucose monitoring (CGM) in conscious unrestrained mice is in development and expected to be offered in the near future. The Core is led by Dr. Peter Havel, DVM, PhD and Co-led by Dr.’s Fawaz Haj, PhD and Oliver Fiehn, PhD. Other Core members include Dr. Mark Huising and Staff Research Associate, James Graham.	SCR_015359
	Energy Balance, Exercise & Behavior Core	Obesity is the result of an inability to maintain energy balance, and changes in energy balance can also be important contributing factors in the etiology of diabetes and other metabolic diseases. A major goal of Core D is to provide investigators with the services necessary to accurately measure the major components of energy balance (energy intake, energy expenditure, body composition, nutrient digestibility) in their mouse models. Core D also provides tests that allow investigators to examine physiological factors that may influence food intake or energy expenditure. A second focus of Core D is to understand the contribution of physical activity to energy balance as well as the beneficial effects of voluntary exercise or treadmill training on metabolism. Behavior is the third component of Core D, with an array of tests for cognitive and behavioral assessment. Core D investigators have complementary expertise in energy metabolism and exercise/muscle biology to provide clients with assistance in all steps of the research process, from designing experiments to analyzing and interpreting data. In addition to offering standard services in our catalog, Core D also works with investigators to design custom tests or develop new tests to meet their research needs.	SCR_015364
	Microbiome & Host Response Core	Mission Statement: To provide clients with a prompt and efficient service to determine how the gut microbiota is contributing to the observed metabolic or gut phenotype. Core Description: The gut is the first site of interaction between ingested nutrients and the host, and plays an important role in the regulation of metabolic homeostasis. The products of digestion provide the stimuli for release of hormones and activation of neural pathways crucial to integrate postprandial gut function, food intake and whole body metabolic function. In addition, there is a growing appreciation of the role of the gut microbial populations in health and disease, especially in metabolic disease and obesity. Thus, determination of gut microbiota and gut function is an important component of metabolic phenotyping. The Microbiome & Host Response Core offers methods to survey the important bacterial taxa: • Metagenomics, especially 16S variable region amplicon sequencing, data analysis and interpretation • Multi-variate analysis • Metabolomic approaches to assess microbial function by measuring levels of microbial metabolites. Priorities of the core include reproducibility and reliability; sample preparation, storage, and handling of small sample volumes; gut region and/or time-dependent differences. The Microbiome & Host Response Core offers methods to determine gut function in metabolic phenotypes: • Measurement of gut permeability (Gut permeability: in vivo, ex vivo Ussing chambers and immunohistochemistry and/or expression of tight junction proteins) • Plasma lipopolysaccharide binding protein (LBP) assay • Inflammatory profiling Other services are offered on a custom basis including measurement of gut transit time, gastric emptying and activity in the gut-brain axis. Future services include transcriptomics of the luminal microbiota and gut tissue of the host.	SCR_015361
	Administrative Core	The Administrative Core is responsible for oversight and coordination of all aspects of the scientific and administrative operations of the MMPC-UCD. This includes providing full customer support services, overseeing management and operation of the Center, and foster interactions and synergism among cores and research projects. The ultimate goal of the Core is to ensure that the Center effectively and efficiently delivers services to its users. The Core will achieve this goal by implementing an administrative plan, establishing and executing a service plan, implementing a business plan, ensuring cooperation and complains with the MMPC Consortium, and overseeing implementation of the Microbiome Research Project. In consultation with the Coordinating and Bioinformatics Unit (CBU), the Core uses MMPC-web-based business tools to facilitate client-provider interaction and help maintain Center budgetary and workflow records. The Core is led by the Center Director (Kent Lloyd) and Scientific Director (John Rutledge), and assisted by a Center Administrator (Suzann Wadsworth) and Project Navigator (Renee Araiza). For more information about the MMPC at UC Davis, or to arrange for phenotyping or your mouse line, contact our Project Navigator at mmpc@ucdavis.edu.	SCR_015362

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THE NATIONAL MMPC MAKES NO REPRESENTATION ABOUT THE SUITABILITY OR ACCURACY OF THE SOFTWARE OR DATA FOR ANY PURPOSE, AND MAKES NO WARRANTIES, EITHER EXPRESS OR IMPLIED, INCLUDING MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE OR THAT THE USE OF THE SOFTWARE OR DATA WILL NOT INFRINGE ANY THIRD PARTY PATENTS, COPYRIGHTS, TRADEMARKS, OR OTHER RIGHTS. THE SOFTWARE AND DATA ARE PROVIDED "AS IS".

The Mouse Metabolic Phenotyping Centers (MMPC) is an NIDDK funded consortium and adheres to the NIH Data Sharing Policy.

MMPC clients make their data freely available whereby MMPC users may freely build upon, enhance and reuse those data for any purpose without restriction. Scholarly citation norms must be followed for content reuse. Please acknowledge the MMPC using the following text: 'The MMPC data used in this manuscript was supported by the NIDDK National Mouse Metabolic Phenotyping Centers (National MMPC, RRID:SCR_008997, www.mmpc.org)'. To cite specific MMPC centers, please use the appropriate RRID available from the MMPC website (https://www.mmpc.org/shared/centers.aspx).

Please note that the acknowledgment text includes a Research Resource Identifier (RRID) for the MMPC CU and Centers. Reproducibility is one of the corner stones of effective, open and transparent biomedical published research. However, too often, resources (e.g. model organisms, antibodies, and tools) are not reported with adequate detail to ensure others can replicate or expand upon the published results. The Research Resource Identification Initiative (#RII) seeks to change these limitations in reporting by the use of unique Research Resource Identifiers (RRIDs). This initiative is designed to encourage authors to provide identification of the types of resources used in their research by adding a globally unique accession number to the resources described in the their manuscripts. These identifiers, called RRIDs, will allow authors to cite the resources that they use in their manuscripts. RRIDs allow for easy tracking of all papers that have used the same resource making it easy to access how the same resources works in other scenarios.

It is expected that MMPC users follow scholarly citation norms, giving credit to fellow scholars when accessing/using protocols and data, including data derived by MMPC (such as summary data) and any plots, tables or screenshots depicting those data.

It is possible for invalid or incomplete results to be presented on the MMPC web site due to software bugs, data problems, or artifacts of human error. Data sets are not necessarily static; we reserve the right to post corrections and updates as needed.

Prohibited use. Data contributors and data users may not use MMPC in any unlawful manner, or in any manner that could impair MMPC services, security or functionality. Automated usage (webcrawlers and similar) must observe each page's "meta robots" html tags and space requests by ≥ 2 seconds. We reserve the right to block any IP associated with what we consider to be excessive or abusive usage patterns, and/or to take any action we deem necessary.

The MMPC is a National Institutes of Health-sponsored resource that provides experimental testing services to scientists studying diabetes, obesity, diabetic complications, and other metabolic diseases in mice.

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