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Catalog Item
Fatty acid profiles of lipid esters by gas liquid chromatography
Common Name(s): Lipid Analysis

CenterVanderbilt University School of Medicine
Center CoreAnalytical Resources Core
Test No.V3072
Test PriceInquire for Pricing. Analytical services are provided as part of in vivo projects.
DescriptionTotal lipids are extracted and lipid classes separated by TLC as described
above. Lipid ester spots are scraped from the plates and methylated. Fatty acids
of lipid esters can be methylated without removal of the lipid from the silica
gel. However, in some applications, we have found it advantageous to elute the
lipid from the silica gel prior to methylation. The fatty acid profile of the
lipid class is also determined. By this method total lipid mass and fatty acid
profile for each lipid is determined.
DataData will be collected for this catalog item
KeywordsGCMS, lipids, metabolism
Tissues



Catalog Groups



Phenotype Assays


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