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Experiment

Circulating insulin levels in B6 mice treated with letrozole
Summary Data Summary
Investigator Thackray, Varykina
Description Letrozole treatment of pubertal female mice results in hallmarks of PCOS
including elevated androgens, anovulation, polycystic ovaries and a metabolic
phenotype.

Puberal (4 week old) female mice were implanted with a placebo or letrozole
pellet (50 ug/day) and treated for 5 weeks. The antibiotics were administered
via mixing it into their drinking water. The antibiotic water was made via the
dosage of 1g/L of Ampicilin, 0.5g/L of Vancomycin, and 1g/L of Neomycin. Mice
had ad lib access to food and water. Mice were sacrificed with isoflurance and
blood was collected via the vena cava and spun down to collect serum.

Applicable research area(s): Metabolism
Status Completed
Public Release 6/13/2020
Animal Age Measured In: week(s) post-natal (w)
Flags has-data-flagSame Strain
Data Analysis
TypeCount
Animals32
Experimental Conditions9
Catalog Items1
Curation Info (# flags)1
Phenotype Assays1
Phenotype Measurements32
Histology Images0
Publications0
Phenotypes1


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6N
32
0
0

Experimental Factors
 Categorical Values
Name / AbbreviationDescription

Experimental Factor: Drug Administered

 Ampicillin, Neomycin, Vancomycin
1g/L of Ampicillin, 1g/L of Neomycin, and 0.5g/L of Vancomycin mixed in water
 Letrozole
Letrozole (INN, trade name Femara) is an oral non-steroidal aromatase inhibitor for the treatment of hormonally-responsive breast cancer after surgery. These are continuous release pellets.
 Vehicle
Vehicle - Control

Experimental Factor: Drug Delivery Route

 Drinking Water
The drug was delivered in the drinking water.
 SC
The drug was delivered subcutaneously.

Experimental Factor: Drug Dose (AU)

 Drug Dose
A numerical value indicating the dose of a drug administered. The unit of measure is indicated in the Drug Dose Units categorical value that accompanies this experimental condition.

Experimental Factor: Drug Dose Units

 µg/day
micrograms per day
 mg/kg/d
milligrams per kilograms per day

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Fast Duration (hr)

 fast duration
Length of time the animal was fasted

Experimental Factor: Feeding Group

 Fasted
Animals are deprived of food but not water.

Experimental Factor: Mouse Diet

 Teklad - 7004
Rodent diet with 29% kcal fat, 51% kcal carbohydrate, 20% kcal protein

Experimental Factor: Tissue Source

 serum
serum


Phenotype Assays Add / Edit



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