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Experiment

Oxymax/Treadmill Order #3605 9/18/12
Summary Data Summary
Investigator Baldwin, Scott
Description Energy expenditure measured by indirect calorimetry in the Oxymax by Columbus
Instruments. Mice were trained to run on the treadmill by running them on a
treadmill for 10 minutes at 10 m/min 2 to 3 days prior to the experiment. On the
day of the experiment mice were placed in the treadmill attached to the oxymax.
The treadmill was turned off for 30 minutes to take readings of the animal
rested. Mice were then run on a treadmill at gradually increasing speeds until
exhaustion. When the mice became exhausted the treadmill was turned off and
recordings were taken for another 30 minutes. Speeds were increased by 4 m/min
every 3 minutes, with the starting speed set to 10 m/min.

Male and Female (C57BL/6J-Ndrg4tm1Bald) and (C57BL/6J-Ndrg4tm1Bald
Tg(MHC-Cre))mice.
Mice were fed a chow diet (LabDiets 5001).

Purpose of the experiment: This experiment will determine whether the groups of
mice have different energy expenditure and different exercise capacity.

Applicable Research Areas: Metabolism , Body weight regulation, energy
expenditure.
Status Completed
Public Release 9/18/2014
Animal Age Measured In: day(s) post-natal (d)
Data Analysis
TypeCount
Animals12
Experimental Conditions2
Catalog Items2
Curation Info (# flags)0
Phenotype Assays8
Phenotype Measurements96
Histology Images0
Publications0


Animals

Strain NameCommon NameFemalesMalesUnknown
C57BL/6-Ndrg4lox/lox
3
3
0
C57BL/6-Ndrg4lox/lox Tg(MHC-Cre)
1
5
0


Experimental Factors
 Name / AbbreviationDescription

Experimental Factor: Experimental Group

 Control
This animal belongs to the control group for the experiment.
 Experiment
This animal belongs to experimental group that is homozygous for gene manipulations.

Experimental Factor: Treadmill Speed (m/min)

 Treadmill_Speed
The speed of the treadmill used during an energy expenditure experiment



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