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Publication
Creation and validation of 3D-printed head molds for stereotaxic injections of
neonatal mouse brains.
Authors Chervonski E, Brockman AA, Khurana R, Chen Y, Greenberg S, Hay MS, Luo Y, Miller
J, Patelis D, Whitney SK, Walker M, Ihrie RA
Submitted By Submitted Externally on 12/3/2021
Status Published
Journal Journal of neuroscience methods
Year 2021
Date Published 8/1/2021
Volume : Pages 360 : 109255
PubMed Reference 34139267
Abstract An increasing number of rodent model systems use injection of DNA or viral
constructs in the neonatal brain. However, approaches for reliable positioning
and stereotaxic injection at this developmental stage are limited, typically
relying on handheld positioning or molds that must be re-aligned for use in a
given laboratory., A complete protocol and open-source software pipeline for
generating 3D-printed head molds derived from a CT scan of a neonatal mouse head
cast, together with a universal adapter that can be placed on a standard
stereotaxic stage., A series of test injections with adenovirus encoding red
fluorescent protein, or Fluorogold, were conducted using original clay molds and
newly generated 3D printed molds. Several metrics were used to compare spread
and localization of targeted injections., The new method of head mold generation
gave comparable results to the field standard, but also allowed the rapid
generation of additional copies of each head mold with standardized positioning
of the head each time., This 3D printing pipeline can be used to efficiently
develop a series of head molds with standardized injection coordinates across
multiple laboratories. More broadly, this pipeline can easily be adapted to
other perinatal ages or species.




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