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Publication
Mfge8 attenuates human gastric antrum smooth muscle contractions.
Authors Li W, Olseen A, Xie Y, Alexandru C, Outland A, Herrera AF, Syder AJ, Wykosky J,
Perrino BA
Submitted By Submitted Externally on 12/3/2021
Status Published
Journal Journal of muscle research and cell motility
Year 2021
Date Published 6/1/2021
Volume : Pages 42 : 219 - 231
PubMed Reference 34085177
Abstract Coordinated gastric smooth muscle contraction is critical for proper digestion
and is adversely affected by a number of gastric motility disorders. In this
study we report that the secreted protein Mfge8 (milk fat globule-EGF factor 8)
inhibits the contractile responses of human gastric antrum muscles to
cholinergic stimuli by reducing the inhibitory phosphorylation of the MYPT1
(myosin phosphatase-targeting subunit (1) subunit of MLCP (myosin light chain
phosphatase), resulting in reduced LC20 (smooth muscle myosin regulatory light
chain (2) phosphorylation. Mfge8 reduced the agonist-induced increase in the
F-actin/G-actin ratios of ß-actin and ?-actin1. We show that endogenous Mfge8 is
bound to its receptor, a8ß1 integrin, in human gastric antrum muscles,
suggesting that human gastric antrum muscle mechanical responses are regulated
by Mfge8. The regulation of gastric antrum smooth muscles by Mfge8 and a8
integrin functions as a brake on gastric antrum mechanical activities. Further
studies of the role of Mfge8 and a8 integrin in regulating gastric antrum
function will likely reveal additional novel aspects of gastric smooth muscle
motility mechanisms.




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