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Publication
Group B Streptococcus cpsE Is Required for Serotype V Capsule Production and
Aids in Biofilm Formation and Ascending Infection of the Reproductive Tract
during Pregnancy.
Authors Noble K, Lu J, Guevara MA, Doster RS, Chambers SA, Rogers LM, Moore RE, Spicer
SK, Eastman AJ, Francis JD, Manning SD, Rajagopal L, Aronoff DM, Townsend SD,
Gaddy JA
Submitted By Submitted Externally on 12/3/2021
Status Published
Journal ACS infectious diseases
Year 2021
Date Published 9/1/2021
Volume : Pages 7 : 2686 - 2696
PubMed Reference 34076405
Abstract Group B Streptococcus (GBS) is an encapsulated Gram-positive pathogen that
causes ascending infections of the reproductive tract during pregnancy. The
capsule of this organism is a critical virulence factor that has been implicated
in a variety of cellular processes to promote pathogenesis. Primarily comprised
of carbohydrates, the GBS capsule and its synthesis is driven by the capsule
polysaccharide synthesis (cps) operon. The cpsE gene within this operon encodes
a putative glycosyltransferase that is responsible for the transfer of a Glc-1-P
from UDP-Glc to an undecaprenyl lipid molecule. We hypothesized that the cpsE
gene product is important for GBS virulence and ascending infection during
pregnancy. Our work demonstrates that a GBS cpsE mutant secretes fewer
carbohydrates, has a reduced capsule, and forms less biofilm than the wild-type
parental strain. We show that, compared to the parental strain, the ?cpsE
deletion mutant is more readily taken up by human placental macrophages and has
a significantly attenuated ability to invade and proliferate in the mouse
reproductive tract. Taken together, these results demonstrate that the cpsE gene
product is an important virulence factor that aids in GBS colonization and
invasion of the gravid reproductive tract.




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