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Publication
High-fat diet induces obesity in adult mice but fails to develop pre-penile and
penile vascular dysfunction.
Authors Odom MR, Hunt TC, Pak ES, Hannan JL
Submitted By Submitted Externally on 12/3/2021
Status Published
Journal International journal of impotence research
Year 2021
Date Published 5/1/2021
Volume : Pages Not Specified : Not Specified
PubMed Reference 33947973
Abstract Obesity can lead to cardiovascular disease, diabetes, and erectile dysfunction
(ED), which decreases overall quality of life. Mechanisms responsible for
obesity-induced ED are unknown. Current mouse models of high-fat diet
(HFD)-induced obesity yield conflicting results. Genetic variants among common
"wild type" strains may explain contradictory data. Adult male C57BL/6N and 6J
mice were fed a 45% HFD for 12 weeks. Weekly food intake, weight gain, and
body-fat percentage were measured. After 12 weeks, ex vivo vascular reactivity
was measured in aortas, internal pudendal arteries, and penises. We assessed
smooth muscle contractility, endothelial-dependent and -independent relaxation,
and penile neurotransmitter-mediated relaxation. C57BL/6N mice developed greater
obesity and glucose sensitivity compared to C57BL/6J mice. Aortas from both
strains that fed a HFD had decreased contraction, yet contraction was unchanged
in HFD pudendal arteries and penises. Interestingly, endothelial-dependent and
-independent relaxation was unchanged in both systemic and penile vasculature.
Likewise, HFD did not impair penile neurotransmitter-mediated relaxation. Both
strains fed 12 weeks of HFD-developed obese phenotypes. However, HFD did not
impair pre-penile or penile smooth muscle vasoreactivity as demonstrated in
previous studies, suggesting that this preclinical model does not accurately
represent the clinical phenotype of obesity-induced ED.




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