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Publication
Multiple interactions of the oncoprotein transcription factor MYC with the
SWI/SNF chromatin remodeler.
Authors Woodley CM, Romer AS, Wang J, Guarnaccia AD, Elion DL, Maxwell JN, Guerrazzi K,
McCann TS, Popay TM, Matlock BK, Flaherty DK, Lorey SL, Liu Q, Tansey WP,
Weissmiller AM
Submitted By Submitted Externally on 12/3/2021
Status Published
Journal Oncogene
Year 2021
Date Published 5/1/2021
Volume : Pages 40 : 3593 - 3609
PubMed Reference 33931740
Abstract The SNF5 subunit of the SWI/SNF chromatin remodeling complex has been shown to
act as a tumor suppressor through multiple mechanisms, including impairing the
ability of the oncoprotein transcription factor MYC to bind chromatin. Beyond
SNF5, however, it is unknown to what extent MYC can access additional SWI/SNF
subunits or how these interactions affect the ability of MYC to drive
transcription, particularly in SNF5-null cancers. Here, we report that MYC
interacts with multiple SWI/SNF components independent of SNF5. We show that MYC
binds the pan-SWI/SNF subunit BAF155 through the BAF155 SWIRM domain, an
interaction that is inhibited by the presence of SNF5. In SNF5-null cells, MYC
binds with remaining SWI/SNF components to essential genes, although for a
purpose that is distinct from chromatin remodeling. Analysis of MYC-SWI/SNF
target genes in SNF5-null cells reveals that they are associated with core
biological functions of MYC linked to protein synthesis. These data reveal that
MYC can bind SWI/SNF in an SNF5-independent manner and that SNF5 modulates
access of MYC to core SWI/SNF complexes. This work provides a framework in which
to interrogate the influence of SWI/SNF on MYC function in cancers in which
SWI/SNF or MYC are altered.




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