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Publication
Glucoregulation and coping behavior after chronic stress in rats: Sex
differences across the lifespan.
Authors Dearing C, Morano R, Ptaskiewicz E, Mahbod P, Scheimann JR, Franco-Villanueva A,
Wulsin L, Myers B
Submitted By Submitted Externally on 1/10/2022
Status Published
Journal Hormones and behavior
Year 2021
Date Published 11/1/2021
Volume : Pages 136 : 105060
PubMed Reference 34537487
Abstract The purpose of the current study was to determine how biological sex shapes
behavioral coping and metabolic health across the lifespan after chronic stress.
We hypothesized that examining chronic stress-induced behavioral and endocrine
outcomes would reveal sex differences in the biological basis of susceptibility.
During late adolescence, male and female Sprague-Dawley rats experienced chronic
variable stress (CVS). Following completion of CVS, all rats experienced a
forced swim test (FST) followed 3 days later by a fasted glucose tolerance test
(GTT). The FST was used to determine coping in response to a stressor. Endocrine
metabolic function was evaluated in the GTT by measuring glucose and
corticosterone, the primary rodent glucocorticoid. Rats then aged to 15 months
when the FST and GTT were repeated. In young rats, chronically stressed females
exhibited more passive coping and corticosterone release in the FST.
Additionally, chronically stressed females had elevated corticosterone and
impaired glucose clearance in the GTT. Aging affected all measurements as
behavioral and endocrine outcomes were sex specific. Furthermore, regression
analysis between hormonal and behavioral responses identified associations
depending on sex and stress. Collectively, these data indicate increased female
susceptibility to the effects of chronic stress during adolescence. Further,
translational investigation of coping style and glucose homeostasis may identify
biomarkers for stress-related disorders.




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