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Publication
Postnatal maturation of calcium signaling in islets of Langerhans from neonatal
mice.
Authors West HL, Corbin KL, D'Angelo CV, Donovan LM, Jahan I, Gu G, Nunemaker CS
Submitted By Submitted Externally on 3/9/2022
Status Published
Journal Cell calcium
Year 2021
Date Published 3/1/2021
Volume : Pages 94 : 102339
PubMed Reference 33422769
Abstract Pancreatic islet cells develop mature physiological responses to glucose and
other fuels postnatally. In this study, we used fluorescence imaging techniques
to measure changes in intracellular calcium ([Ca2+]i) to compare islets isolated
from mice on postnatal days 0, 4, and 12 with islets from adult CD-1 mice. In
addition, we used publicly available RNA-sequencing data to compare expression
levels of key genes in ß-cell physiology with [Ca2+]i data across these ages. We
show that islets isolated from mice on postnatal day 0 displayed elevated
[Ca2+]i in basal glucose (=4?mM) but lower [Ca2+]i responses to stimulation by
12-20?mM glucose compared to adult. Neonatal islets displayed more adult-like
[Ca2+]i in basal glucose by day 4 but continued to show lower [Ca2+]i responses
to 16 and 20?mM glucose stimulation up to at least day 12. A right shift in
glucose sensing (EC50) correlated with lower
fragment-per-kilobase-of-transcript-per-million-reads-mapped (FPKM) of Slc2a2
(glut2) and Actn3 and increased FPKM for Galk1 and Nupr1. Differences in [Ca2+]i
responses to additional stimuli were also observed. Calcium levels in the
endoplasmic reticulum were elevated on day 0 but became adult-like by day 4,
which corresponded with reduced expression in Atp2a2 (SERCA2) and novel
K+-channel Ktd17, increased expression of Pml, Wfs1, Thada, and Herpud1, and
basal [Ca2+]i maturing to adult levels. Ion-channel activity also matured
rapidly, but RNA sequencing data mining did not yield strong leads. In
conclusion, the maturation of islet [Ca2+]i signaling is complex and
multifaceted; several possible gene targets were identified that may participate
in this process.




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