mmpc-logo mmpc-logo
twitter-logo    bluesky-logo
| Create Account | login
Publication
Nutritionally Responsive PMv DAT Neurons Are Dynamically Regulated During
Pubertal Transition.
Authors Sáenz de Miera C, Bellefontaine N, Silveira MA, Fortin CN, Zampieri TT, Donato
J, Williams KW, Mendes-da-Silva C, Heikkinen L, Broberger C, Frazao R, Elias CF
Submitted By Submitted Externally on 8/28/2025
Status Published
Journal FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Year 2025
Date Published 7/1/2025
Volume : Pages 39 : e70755
PubMed Reference 40577056
Abstract Pubertal development is tightly regulated by energy balance. The crosstalk
between metabolism and reproduction is orchestrated by complex neural networks,
and leptin action in the hypothalamus plays a critical role. The ventral
premammillary nucleus (PMv) leptin receptor (LepRb) neurons act as an essential
relay for leptin action on reproduction. Here, we show that mouse PMv cells
expressing the dopamine transporter (DAT) gene, Slc6a3 (PMvDAT), form a novel
subpopulation of LepRb neurons. Virtually, all PMvDAT neurons expressed Lepr
mRNA and responded to acute leptin treatment. Electrophysiological recordings
from DATCRE; tdTomato mice showed that PMvDAT cells in prepubertal females have
a hyperpolarized resting membrane potential compared to diestrous females.
Slc6a3 mRNA expression in the PMv was higher in prepubertal than in adult
females. In prepubertal females, Slc6a3 mRNA expression was higher in
overnourished females from small size litters than in controls. Prepubertal
Lepob females showed decreased PMv Slc6a3 mRNA expression that recovered to
control levels after 3?days of leptin injections. Using a tracer adenoassociated
virus in the PMv of adult DATCre; Kiss1hrGFP females, we observed PMvDAT
projections in the anteroventral periventricular and periventricular nucleus
(AVPV/PeN), surrounding Kiss1hrGFP neurons, a population critical for sexual
maturation and positive estrogen feedback in females. The DATCRE; tdTomato
projections to the AVPV were denser in adult than in prepubertal females. In
adults, they are in close apposition to tyrosine hydroxylase neurons. Overall,
these findings suggest that the DAT expressing PMvLepRb subpopulation play a
role in leptin regulation of sexual maturation via actions on AVPV
kisspeptin/tyrosine hydroxylase neuron.




Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.