mmpc-logo mmpc-logo
twitter-logo    bluesky-logo
| Create Account | login
Publication
The MafA transcription factor becomes essential to islet ß-cells soon after
birth.
Authors Hang Y, Yamamoto T, Benninger RK, Brissova M, Guo M, Bush W, Piston DW, Powers
AC, Magnuson M, Thurmond DC, Stein R
Submitted By Roland Stein on 8/5/2014
Status Published
Journal Diabetes
Year 2014
Date Published 6/1/2014
Volume : Pages 63 : 1994 - 2005
PubMed Reference 24520122
Abstract The large Maf transcription factors, MafA and MafB, are expressed with distinct
spatial-temporal patterns in rodent islet cells. Analysis of Mafa(-/-) and
pancreas-specific Mafa(?panc) deletion mutant mice demonstrated a primary role
for MafA in adult ß-cell activity, different from the embryonic importance of
MafB. Our interests here were to precisely define when MafA became functionally
significant to ß-cells, to determine how this was affected by the brief period
of postnatal MafB production, and to identify genes regulated by MafA during
this period. We found that islet cell organization, ß-cell mass, and ß-cell
function were influenced by 3 weeks of age in Mafa(?panc) mice and compromised
earlier in Mafa(?panc);Mafb(+/-) mice. A combination of genome-wide microarray
profiling, electron microscopy, and metabolic assays were used to reveal
mechanisms of MafA control. For example, ß-cell replication was produced by
actions on cyclin D2 regulation, while effects on granule docking affected
first-phase insulin secretion. Moreover, notable differences in the genes
regulated by embryonic MafB and postnatal MafA gene expression were found. These
results not only clearly define why MafA is an essential transcriptional
regulator of islet ß-cells, but also why cell maturation involves coordinated
actions with MafB.




Menu

Home
Contact
About MMPC
Animal Husbandry
Tests Data
Search Data
Analysis
Clients
MMPC Centers

Newsletter

Interested in receiving MMPC News?
twitter-logo Mouse Phenotyping
@NationalMMPC



2017 National MMPC. All Rights Reserved.