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Publication
Metabolic phenotyping of an adoptive transfer mouse model of experimental
colitis and impact of dietary fish oil intake.
Authors Martin FP, Lichti P, Bosco N, Brahmbhatt V, Oliveira M, Haller D, Benyacoub J
Submitted By Submitted Externally on 7/24/2015
Status Published
Journal Journal of proteome research
Year 2015
Date Published 4/3/2015
Volume : Pages 14 : 1911 - 1919
PubMed Reference 25751005
Abstract Inflammatory bowel diseases are acute and chronic disabling inflammatory
disorders with multiple complex etiologies that are not well-defined. Chronic
intestinal inflammation has been linked to an energy-deficient state of gut
epithelium with alterations in oxidative metabolism. Plasma-, urine-, stool-,
and liver-specific metabonomic analyses are reported in a naïve T cell adoptive
transfer (AT) experimental model of colitis, which evaluated the impact of
long-chain n-3 polyunsaturated fatty acid (PUFA)-enriched diet. Metabolic
profiles of AT animals and their controls under chow diet or fish oil
supplementation were compared to describe the (i) consequences of inflammatory
processes and (ii) the differential impact of n-3 fatty acids. Inflammation was
associated with higher glycoprotein levels (related to acute-phase response) and
remodeling of PUFAs. Low triglyceride levels and enhanced PUFA levels in the
liver suggest activation of lipolytic pathways that could lead to the observed
increase of phospholipids in the liver (including plasmalogens and
sphingomyelins). In parallel, the increase in stool excretion of most amino
acids may indicate a protein-losing enteropathy. Fecal content of glutamine was
lower in AT mice, a feature exacerbated under fish oil intervention that may
reflect a functional relationship between intestinal inflammatory status and
glutamine metabolism. The decrease in Krebs cycle intermediates in urine
(succinate, a-ketoglutarate) also suggests a reduction in the glutaminolytic
pathway at a systemic level. Our data indicate that inflammatory status is
related to this overall loss of energy homeostasis.




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