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Publication
Defective insulin secretory response to intravenous glucose in C57Bl/6J compared
to C57Bl/6N mice.
Authors Fergusson G, Ethier M, Guévremont M, Chrétien C, Attané C, Joly E, Fioramonti X,
Prentki M, Poitout V, Alquier T
Submitted By Submitted Externally on 7/24/2015
Status Published
Journal Molecular metabolism
Year 2014
Date Published 12/1/2014
Volume : Pages 3 : 848 - 854
PubMed Reference 25506550
Abstract The C57Bl/6J (Bl/6J) mouse is the most widely used strain in metabolic research.
This strain carries a mutation in nicotinamide nucleotide transhydrogenase
(Nnt), a mitochondrial enzyme involved in NADPH production, which has been
suggested to lead to glucose intolerance and beta-cell dysfunction. However,
recent reports comparing Bl/6J to Bl/6N (carrying the wild-type Nnt allele)
under normal diet have led to conflicting results using glucose tolerance tests.
Thus, we assessed glucose-stimulated insulin secretion (GSIS), insulin
sensitivity, clearance and central glucose-induced insulin secretion in Bl/6J
and N mice using gold-standard methodologies.




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