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Publication
A review of standardized metabolic phenotyping of animal models.
Authors Rozman J, Klingenspor M, Hrabe de Angelis M
Submitted By Submitted Externally on 7/24/2015
Status Published
Journal Mammalian genome : official journal of the International Mammalian Genome Society
Year 2014
Date Published 10/1/2014
Volume : Pages 25 : 497 - 507
PubMed Reference 25199945
Abstract Metabolic phenotyping of genetically modified animals aims to detect new
candidate genes and related metabolic pathways that result in dysfunctional
energy balance regulation and predispose for diseases such as obesity or type 2
diabetes mellitus. In this review, we provide a comprehensive overview on the
technologies available to monitor energy flux (food uptake, bomb calorimetry of
feces and food, and indirect calorimetry) and body composition (qNMR, DXA, and
MRI) in animal models for human diseases with a special focus on phenotyping
methods established in genetically engineered mice. We use an energy flux model
to illustrate the principles of energy allocation, describe methodological
aspects how to monitor energy balance, and introduce strategies for data
analysis and presentation.




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