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Publication
MRI measurement of blood-brain barrier transport with a rapid acquisition
refocused echo (RARE) method.
Authors Walton JH, Ng KF, Anderson SE, Rutledge JC
Submitted By Submitted Externally on 11/3/2015
Status Published
Journal Biochemical and biophysical research communications
Year 2015
Date Published 8/1/2015
Volume : Pages 463 : 479 - 82
PubMed Reference 25998382
Abstract Dynamic Contrast Enhanced (DCE) MRI is increasingly being used to assess changes
in capillary permeability. Most quantitative techniques used to measure
capillary permeability are based on the Fick equation that requires measurement
of signal reflecting both plasma and tissue concentrations of the solute being
tested. To date, most Magnetic Resonance Imaging (MRI) methods for acquiring
appropriate data quickly rely on gradient recalled echo (GRE) type acquisitions,
which work well in clinical low field settings. However, acquiring this type of
data on high field small animal preclinical MRIs is problematic due to
geometrical distortions from susceptibility mismatch. This problem can be
exacerbated when using small animal models to measure blood brain barrier (BBB)
permeability, where precise sampling from the superior sagittal sinus (SSS) is
commonly used to determine the plasma concentration of the contrast agent. Here
we present results demonstrating that a standard saturation recovery rapid
acquisition refocused echo (RARE) method is capable of acquiring T1 maps with
good spatial and temporal resolution for Patlak analysis (Patlak, 1983) to
assess changes in BBB Gd-DTPA permeability following middle cerebral artery
occlusion with reperfusion in the rat. This method limits known problems with
magnetic susceptibility mismatch and may thus allow greater accuracy in BBB
permeability measurement in small animals.




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