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Publication
Characterizing blood-brain barrier perturbations after exposure to human
triglyceride-rich lipoprotein lipolysis products using MRI in a rat model.
Authors Ng KF, Anderson S, Mayo P, Hnin Aung, Walton JH, Rutledge JC
Submitted By Submitted Externally on 11/3/2015
Status Published
Journal Magnetic Resonance in Medicine
Year 2015
Date Published
Volume : Pages Not Specified : Not Specified
PubMed Reference 26485349
Abstract Previous studies indicated hyperlipidemia may be a risk factor for Alzheimer's
disease, but the contributions of postprandial triglyceride-rich lipoprotein
(TGRL) are not known. In this study, changes in blood-brain barrier diffusional
transport following exposure to human TGRL lipolysis products were studied using
MRI in a rat model., Male Sprague-Dawley rats (~180-250 g) received an i.v.
injection of lipoprotein lipase (LpL)-hydrolyzed TGRL (n?=?8, plasma
concentration ˜ 150 mg human TGRL/dL). Controls received i.v. injection of
either saline (n?=?6) or LpL only (n?=?6). The (1) H longitudinal relaxation
rate R1 ?=?1/T1 was measured over 18 min using a rapid-acquired refocus-echo
(RARE) sequence after each of three injections of the contrast agent Gd-DTPA.
Patlak plots were generated for each pixel yielding blood-to-brain transfer
coefficients, Ki , chosen for best fit to impermeable, uni-directional influx or
bi-directional flux models using the F-test., Analysis from a 2-mm slice, 2-mm
rostral to the bregma showed a 275% increase of mean Ki during the first 20 min
after infusion of human TGRL lipolysis product that differed significantly
compared with saline and LpL controls. This difference disappeared by 40 min
mark., These results suggest human TGRL lipolysis products can lead to a
transient increase in rat BBB permeability. Magn Reson Med, 2015. © 2015 The
Authors. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on
behalf of International Society for Magnetic Resonance in Medicine. This is an
open access article under the terms of the Creative Commons Attribution
NonCommercial License, which permits use, distribution and reproduction in any
medium, provided the original work is properly cited and is not used for
commercial purposes.




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