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Publication
A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal
bodies.
Authors Jao LE, Akef A, Wente SR
Submitted By Submitted Externally on 1/18/2017
Status Published
Journal Molecular biology of the cell
Year 2017
Date Published 1/1/2017
Volume : Pages 28 : 120 - 127
PubMed Reference 28035044
Abstract Control of organellar assembly and function is critical to eukaryotic
homeostasis and survival. Gle1 is a highly conserved regulator of RNA-dependent
DEAD-box ATPase proteins, with critical roles in both mRNA export and
translation. In addition to its well-defined interaction with nuclear pore
complexes, here we find that Gle1 is enriched at the centrosome and basal body.
Gle1 assembles into the toroid-shaped pericentriolar material around the mother
centriole. Reduced Gle1 levels are correlated with decreased pericentrin
localization at the centrosome and microtubule organization defects. Of
importance, these alterations in centrosome integrity do not result from loss of
mRNA export. Examination of the Kupffer's vesicle in Gle1-depleted zebrafish
revealed compromised ciliary beating and developmental defects. We propose that
Gle1 assembly into the pericentriolar material positions the DEAD-box protein
regulator to function in localized mRNA metabolism required for proper
centrosome function.




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