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Publication
Antibiotics Suppress Activation of Intestinal Mucosal Mast Cells and Reduce
Dietary Lipid Absorption in Sprague-Dawley Rats.
Authors Sato H, Zhang LS, Martinez K, Chang EB, Yang Q, Wang F, Howles PN, Hokari R,
Miura S, Tso P
Submitted By Submitted Externally on 4/17/2017
Status Published
Journal Gastroenterology
Year 2016
Date Published 11/1/2016
Volume : Pages 151 : 923 - 932
PubMed Reference 27436071
Abstract The gut microbiota affects intestinal permeability and mucosal mast cells (MMCs)
responses. Activation of MMCs has been associated with absorption of dietary
fat. We investigated whether the gut microbiota contributes to the fat-induced
activation of MMCs in rats, and how antibiotics might affect this process.,
Adult male Sprague-Dawley rats were given streptomycin and penicillin for 4 days
(n = 6-8) to reduce the abundance of their gut flora, or normal drinking water
(controls, n = 6-8). They underwent lymph fistula surgery and after an overnight
recovery were given an intraduodenal bolus of intralipid. We collected
intestinal tissues and lymph fluid and assessed activation of MMCs, intestinal
permeability, and fat transport parameters., Compared with controls, intestinal
lymph from rats given antibiotics had reduced levels of mucosal mast cell
protease II (produced by MMCs) and decreased activity of diamine oxidase
(produced by enterocytes) (P < .05). Rats given antibiotics had reduced
intestinal permeability in response to dietary lipid compared with controls (P <
.01). Unexpectedly, antibiotics also reduced lymphatic transport of
triacylglycerol and phospholipid (P < .01), concomitant with decreased levels of
mucosal apolipoproteins B, A-I, and A-IV (P < .01). No differences were found in
intestinal motility or luminal pancreatic lipase activity between rats given
antibiotics and controls. These effects were not seen with an acute dose of
antibiotics or 4 weeks after the antibiotic regimen ended., The intestinal
microbiota appears to activate MMCs after the ingestion of fat in rats; this
contributes to fat-induced intestinal permeability. We found that the gut
microbiome promotes absorption of lipid, probably by intestinal production of
apolipoproteins and secretion of chylomicrons.




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