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Publication
In Vivo Imaging of Retinal Hypoxia Using HYPOX-4-Dependent Fluorescence in a
Mouse Model of Laser-Induced Retinal Vein Occlusion (RVO).
Authors Uddin MI, Jayagopal A, McCollum GW, Yang R, Penn JS
Submitted By Ashwath Jayagopal on 8/17/2017
Status Published
Journal Investigative ophthalmology & visual science
Year 2017
Date Published 7/1/2017
Volume : Pages 58 : 3818 - 3824
PubMed Reference 28750413
Abstract To demonstrate the utility of a novel in vivo molecular imaging probe, HYPOX-4,
to detect and image retinal hypoxia in real time, in a mouse model of retinal
vein occlusion (RVO)., Retinal vein occlusion was achieved in adult mice by
photodynamic retinal vein thrombosis (PRVT). One or two major retinal vein(s)
was/were occluded in close proximity to the optic nerve head (ONH). In vivo
imaging of retinal hypoxia was performed using, HYPOX-4, an imaging probe
developed by our laboratory. Pimonidazole-adduct immunostaining was performed
and used as a standard ex vivo method for the detection of retinal hypoxia in
this mouse RVO model. The retinal vasculature was imaged using fluorescein
angiography (FA) and isolectin B4 staining. Retinal thickness was assessed by
spectral-domain optical coherence tomography (SD-OCT) analysis., By application
of the standard ex vivo pimonidazole-adduct immunostaining technique, retinal
hypoxia was observed within 2 hours post-PRVT. The observed hypoxic retinal
areas depended on whether one or two retinal vein(s) was/were occluded. Similar
areas of hypoxia were imaged in vivo using HYPOX-4. Using OCT, retinal edema was
observed immediately post-PRVT induction, resolving 8 days later. Nominal
preretinal neovascularization was observed at 10 to 14 days post-RVO., HYPOX-4
is an efficient probe capable of imaging retinal hypoxia in vivo, in RVO mice.
Future studies will focus on its use in correlating retinal hypoxia to the onset
and progression of ischemic vasculopathies.




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