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Publication
Theranostic Gold Nanoantennas for Simultaneous Multiplexed Raman Imaging of
Immunomarkers and Photothermal Therapy.
Authors Webb JA, Ou YC, Faley S, Paul EP, Hittinger JP, Cutright CC, Lin EC, Bellan LM,
Bardhan R
Submitted By Submitted Externally on 9/13/2017
Status Published
Journal ACS omega
Year 2017
Date Published 7/1/2017
Volume : Pages 2 : 3583 - 3594
PubMed Reference 28782050
Abstract In this study, we demonstrate the theranostic capability of actively targeted,
site-specific multibranched gold nanoantennas (MGNs) in triple-negative breast
cancer (TNBC) cells in vitro. By utilizing multiplexed surface-enhanced Raman
scattering (SERS) imaging, enabled by the narrow peak widths of Raman
signatures, we simultaneously targeted immune checkpoint receptor programmed
death ligand 1 (PDL1) and the epidermal growth factor receptor (EGFR)
overexpressed in TNBC cells. A 1:1 mixture of MGNs functionalized with anti-PDL1
antibodies and Raman tag 5,5-dithio-bis-(2-nitrobenzoic acid) (DTNB) and MGNs
functionalized with anti-EGFR antibodies and Raman tag para-mercaptobenzoic acid
(pMBA) were incubated with the cells. SERS imaging revealed a cellular traffic
map of MGN localization by surface binding and receptor-mediated endocytosis,
enabling targeted diagnosis of both biomarkers. Furthermore, cells incubated
with anti-EGFR-pMBA-MGNs and illuminated with an 808 nm laser for 15 min at 4.7
W/cm(2) exhibited photothermal cell death only within the laser spot (indicated
by live/dead cell fluorescence assay). Therefore, this study not only provides
an optical imaging platform that can track immunomarkers with spatiotemporal
control but also demonstrates an externally controlled light-triggered
therapeutic approach enabling receptor-specific treatment with biocompatible
theranostic nanoprobes.




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