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Publication
In vivo measurement of vocal fold surface resistance.
Authors Mizuta M, Kurita T, Dillon NP, Kimball EE, Garrett CG, Sivasankar MP, Webster
RJ, Rousseau B
Submitted By Submitted Externally on 9/26/2017
Status Published
Journal The Laryngoscope
Year 2017
Date Published 10/1/2017
Volume : Pages 127 : E364 - E370
PubMed Reference 28573762
Abstract A custom-designed probe was developed to measure vocal fold surface resistance
in vivo. The purpose of this study was to demonstrate proof of concept of using
vocal fold surface resistance as a proxy of functional tissue integrity after
acute phonotrauma using an animal model., Prospective animal study., New Zealand
White breeder rabbits received 120 minutes of airflow without vocal fold
approximation (control) or 120 minutes of raised intensity phonation
(experimental). The probe was inserted via laryngoscope and placed on the left
vocal fold under endoscopic visualization. Vocal fold surface resistance of the
middle one-third of the vocal fold was measured after 0 (baseline), 60, and 120
minutes of phonation. After the phonation procedure, the larynx was harvested
and prepared for transmission electron microscopy., In the control group, vocal
fold surface resistance values remained stable across time points. In the
experimental group, surface resistance (X% ± Y% relative to baseline) was
significantly decreased after 120 minutes of raised intensity phonation. This
was associated with structural changes using transmission electron microscopy,
which revealed damage to the vocal fold epithelium after phonotrauma, including
disruption of the epithelium and basement membrane, dilated paracellular spaces,
and alterations to epithelial microprojections. In contrast, control vocal fold
specimens showed well-preserved stratified squamous epithelia., These data
demonstrate the feasibility of measuring vocal fold surface resistance in vivo
as a means of evaluating functional vocal fold epithelial barrier integrity.
Device prototypes are in development for additional testing, validation, and for
clinical applications in laryngology., NA Laryngoscope, 127:E364-E370, 2017.




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