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Publication
Tunable Surface Repellency Maintains Stemness and Redox Capacity of Human
Mesenchymal Stem Cells.
Authors Balikov DA, Crowder SW, Boire TC, Lee JB, Gupta MK, Fenix AM, Lewis HN, Ambrose
CM, Short PA, Kim CS, Burnette DT, Reilly MA, Murthy NS, Kang ML, Kim WS, Sung
HJ
Submitted By Submitted Externally on 11/9/2017
Status Published
Journal ACS applied materials & interfaces
Year 2017
Date Published 7/1/2017
Volume : Pages 9 : 22994 - 23006
PubMed Reference 28621931
Abstract Human bone marrow derived mesenchymal stem cells (hMSCs) hold great promise for
regenerative medicine due to their multipotent differentiation capacity and
immunomodulatory capabilities. Substantial research has elucidated mechanisms by
which extracellular cues regulate hMSC fate decisions, but considerably less
work has addressed how material properties can be leveraged to maintain
undifferentiated stem cells. Here, we show that synthetic culture substrates
designed to exhibit moderate cell-repellency promote high stemness and low
oxidative stress-two indicators of naïve, healthy stem cells-in commercial and
patient-derived hMSCs. Furthermore, the material-mediated effect on cell
behavior can be tuned by altering the molar percentage (mol %) and/or chain
length of poly(ethylene glycol) (PEG), the repellant block linked to hydrophobic
poly(e-caprolactone) (PCL) in the copolymer backbone. Nano- and angstrom-scale
characterization of the cell-material interface reveals that PEG interrupts the
adhesive PCL domains in a chain-length-dependent manner; this prevents hMSCs
from forming mature focal adhesions and subsequently promotes cell-cell
adhesions that require connexin-43. This study is the first to demonstrate that
intrinsic properties of synthetic materials can be tuned to regulate the
stemness and redox capacity of hMSCs and provides new insight for designing
highly scalable, programmable culture platforms for clinical translation.




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