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Publication
Effect of ApoA4 on SERPINA3 mediated by nuclear receptors NR4A1 and NR1D1 in
hepatocytes.
Authors Zhang Y, He J, Zhao J, Xu M, Lou D, Tso P, Li Z, Li X
Submitted By Submitted Externally on 12/5/2017
Status Published
Journal Biochemical and biophysical research communications
Year 2017
Date Published 5/1/2017
Volume : Pages 487 : 327 - 332
PubMed Reference 28412351
Abstract ApoA4 exerts anti-inflammatory effects, but the mechanism remains unclear.
SERPINA3 is a member of the serine proteinase inhibitor gene family, and has
been shown to be involved in anti-inflammation and associated with a number of
human diseases. In this study, we revealed that ApoA4 stimulates the gene
expression of SERPINA3 in mouse hepatocytes both in vivo and in vitro, in a
dose- and time-dependent manner. The transcriptional response of SERPINA3 to
ApoA4 is regulated through the binding of ApoA4 with nuclear receptors NR4A1 and
NR1D1 on the SERPINA3 promoter, which was verified with ChIP, Luciferase
activity assay and RNA interference-mediated NR4A1 or NR1D1 gene knockdown.
These data suggests that ApoA4 transcriptionally induced SERPINA3 expression via
NR1D1 and NR4A1. Our findings may throw light on the function of ApoA4 in
inflammatory responses and acute-phase reactions, as well as the development of
SERPINA3 relative diseases.




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