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Publication
Priming Effect of a Morning Meal on Hepatic Glucose Disposition Later in the
Day.
Authors Moore MC, Smith MS, Farmer B, Kraft G, Shiota M, Williams PE, Cherrington AD
Submitted By Submitted Externally on 1/3/2018
Status Published
Journal Diabetes
Year 2017
Date Published 5/1/2017
Volume : Pages 66 : 1136 - 1145
PubMed Reference 28174290
Abstract We used hepatic balance and tracer ([3H]glucose) techniques to examine the
impact of "breakfast" on hepatic glucose metabolism later in the same day. From
0-240 min, 2 groups of conscious dogs (n = 9 dogs/group) received a duodenal
infusion of glucose (GLC) or saline (SAL), then were fasted from 240-360 min.
Three dogs from each group were euthanized and tissue collected at 360 min. From
360-600 min, the remaining dogs underwent a hyperinsulinemic (4× basal)
hyperglycemic clamp (arterial blood glucose 146 ± 2 mg/dL) with portal GLC
infusion. The total GLC infusion rate was 14% greater in dogs infused with GLC
than in those receiving SAL (AUC360-600min 2,979 ± 296 vs. 2,597 ± 277 mg/kg,
respectively). The rates of hepatic glucose uptake (5.8 ± 0.8 vs. 3.2 ± 0.3 mg ·
kg-1 · min-1) and glycogen storage (4.7 ± 0.6 vs. 2.9 ± 0.3 mg · kg-1 · min-1)
during the clamp were markedly greater in dogs receiving GLC compared with those
receiving SAL. Hepatic glycogen content was ~50% greater, glycogen synthase
activity was ~50% greater, glycogen phosphorylase activity was ~50% lower, and
the amount of phosphorylated glycogen synthase was 34% lower, indicating
activation of the enzyme, in dogs receiving GLC compared with those receiving
SAL. Thus, morning GLC primed the liver to extract and store more glucose in the
presence of hyperinsulinemic hyperglycemia later in the same day, indicating
that breakfast enhances the liver's role in glucose disposal in subsequent
same-day meals.




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